Quantitation of the HIV-1 p24 core protein of a lentiviral vector is an efficient, well-published method for determining lentivirus physical titer. The QuickTiter™ Lentivirus Quantitation Kits provide a convenient method of p24 antigen quantitation.
Figures
Figure 1: Quantitation of GFP Lentiviral Supernatant with the QuickTiter™ Lentivirus Quantitation Kit (HIV p24 ELISA). A GFP lentiviral construct was cotransfected with a packaging mix into 293 cells. The conditioned medium was harvested 48 hours after transfection and used to further infect 293 cells.
Figure 1
Figure 2: Quantitation of GFP Lentiviral Supernatant with the QuickTiter™ Lentivirus Quantitation Kit (HIV p24 ELISA). A GFP lentiviral construct was cotransfected with a packaging mix into 293 cells. The p24 level of the diluted lentiviral supernatant (1:10 dilution) was determined as described in the assay protocol.
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