The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species.
Our OxiSelect™ Protein Carbonyl Assay Kits provide rapid, efficient methods for detection of protein carbonyl residues. The immunoblot format provides a convenient way to compare oxidized and non-oxidized protein fingerprints. The spectrophotometric format is ideal for assay of just a few samples. The ELISA format is perfect for higher throughput and high sensitivity; we have eliminated concentration and precipitation steps allowing greater sample retention. The fluorescence format also provides a convenient system to measure protein carbonyl content- the fluorescence produced is a direct measure of protein carbonyls.
Figure 1: Assay Principle for the OxiSelect™ Protein Oxidation Immunoblot Kit.
Figure 2: Standard Curve Generated with the OxiSelect™ Protein Carbonyl ELISA Kit.