Protein Carbonyl Assays

The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species.

Our OxiSelect™ Protein Carbonyl Assay Kits provide rapid, efficient methods for detection of protein carbonyl residues. The immunoblot format provides a convenient way to compare oxidized and non-oxidized protein fingerprints. The spectrophotometric format is ideal for assay of just a few samples. The ELISA format is perfect for higher throughput and high sensitivity; we have eliminated concentration and precipitation steps allowing greater sample retention. The fluorescence format also provides a convenient system to measure protein carbonyl content- the fluorescence produced is a direct measure of protein carbonyls.

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2. Young, K. et al. (2013). Each to Their Own: Skeletal Muscles of Different Function use Different Biochemical Strategies During Aestivation at High Temperature. J.Exp. Biol. 216:1012-1024 (#STA-310).
3. Fishman, J. et al. (2012). Oxidative Modification of the Intestinal Mucus Layer is a Critical but Unrecognized Component of Trauma Hemorrhagic Shock-Induced gut Barrier Failure. Am J Physiol Gastrointest Liver Physiol. 304:G57-G63 (#STA-310).
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14. Liu, Z. et al. (2007). Hydroxytyrosol Protects Retinal Pigment Epithelia lCells from Acrolein-Induced Oxidative Stress and Mitochondrial Dysfunction. J. Neurochem. 103:2690-2700 (#STA-308).
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28. Kim, S-H. et al. (2008). Protective Effect of Esculetin Against Oxidative Stress-Induced Cell Damage via Scavenging Reactive Oxygen Species. Acta Pharmacol. Sinica 29(11):1319-1326 (#STA-310).
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