Cell Based Assays
Metabolism ResearchOxidative Stress / Damage
Stem Cell Research
Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit.
If you are also studying Cdc42 or RhoA, you may consider one of our economical combination kits.
Figure 1: Small GTPase Activation Assay Principle
Figure 2: Immunoblotting with the Rac Activation Assay. Lane 1: GTPase Immunoblot Positive Control. Lane 2: 293 cell lysate loaded with GDP and incubated with PAK1 PBD Agarose beads. Lane 3: 293 cell lysate loaded with GTPγS and incubated with PAK1 PBD Agarose beads.