Small GTPase Activation AssaysSmall GTPase Research Tools
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- Safe non-radioactive assay format
- Colored agarose beads allow visual check
- Fast results: 1 hour plus electrophoresis/blotting time
| Product Name | Catalog Number | Size | Detection | Qty. | Price |
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| RhoA/Rac1/Cdc42 Activation Assay Combo Kit | STA-405 |
| Rac1/Cdc42 Activation Assay Combo Kit | STA-404 |
| Arf1 Activation Assay | STA-407-1 |
| Arf6 Activation Assay | STA-407-6 |
| Cdc42 Activation Assay | STA-402 |
| Rac1 Activation Assay | STA-401-1 |
| Rac2 Activation Assay | STA-401-2 |
| Ral Activation Assay | STA-408 |
| Ran Activation Assay | STA-409 |
| Rap1 Activation Assay Kit | STA-406-1 |
| Rap2 Activation Assay Kit | STA-406-2 |
| H-Ras Activation Assay Kit | STA-400-H |
| K-Ras Activation Assay Kit | STA-400-K |
| N-Ras Activation Assay Kit | STA-400-N |
| Pan-Ras Activation Assay | STA-400 |
| RhoA Activation Assay | STA-403-A |
| RhoB Activation Assay | STA-403-B |
| RhoC Activation Assay | STA-403-C |
Overview
Our Small GTPase Activation Assays use visible agarose beads to selectively precipitate the active form of the target of interest. The precipitated small GTPase is then detected by Western blot using a target-specific antibody included in the kit.
Figures
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Figure 1: Small GTPase Activation Assay Principle
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Figure 2: Immunoblotting with the Rac Activation Assay. Lane 1: GTPase Immunoblot Positive Control. Lane 2: 293 cell lysate loaded with GDP and incubated with PAK1 PBD Agarose beads. Lane 3: 293 cell lysate loaded with GTPγS and incubated with PAK1 PBD Agarose beads.
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Recent Product Citations
- Wang, J. et al. (2013). DEK Depletion Negatively Regulates Rho/ROCK/MLC Pathway in Non–Small Cell Lung Cancer. Journal of Histochemistry & Cytochemistry. 10.1369/0022155413488120 (#STA-405).
- He, S. et al. (2013). SRGAP1 Is a Candidate Gene for Papillary Thyroid Carcinoma Susceptibility. J. Clin. Endocrinol. Metab. 98:E973-E980 (#STA-402).
- Yuen, H. et al. (2013). RanGTPase: A Candidate for Myc-Mediated Cancer Progression. J Natl Cancer Inst. 105:475-488 (#STA-409).
- Grossmann, A. Et al. (2013). The Small GTPase ARF6 Stimulates β-Catenin Transcriptional Activity During WNT5A-Mediated Melanoma Invasion and Metastasis. Sci. Signal. 6:ra14.
- Quint, P. et al. (2013). Sphingosine 1-Phosphate (S1P) Receptors 1 and 2 Coordinately Induce Mesenchymal Cell Migration through S1P Activation of Complementary Kinase Pathways. J. Biol. Chem. 288:5398-5406 (#STA-405).
- Basu, M. et al. (2012). Wnt/β-Catenin Pathway Is Regulated by PITX2 Homeodomain Protein and Thus Contributes to the Proliferation of Human Ovarian Adenocarcinoma Cell, SKOV-3. J. Biol. Chem. 288:4355-4367 (#STA-403-A).
- Cristante, E. et al. (2013). From the Cover: Feature Article: Identification of an Essential Endogenous Regulator of Blood–Brain Barrier Integrity, and its Pathological and Therapeutic Implications. PNAS. 110:832-841 (#STA-401-1).
- Zhang, L. et al. (2012). A Novel Role of Sphingosine 1-Phosphate Receptor S1pr1 in Mouse Thrombopoiesis. J. Exp. Med. 209: 2165-2181 (#STA-401-1).
- Ohashi, K. et al. (2012). Lung Cancers with Acquired Resistance to EGFR Inhibitors Occasionally Harbor BRAF Gene Mutations but Lack Mutations in KRAS, NRAS, or MEK1. PNAS. 109:E2127-E2133 (#STA-100).
- Tripathi, V. et al. (2012). DLC1 Interaction with α-Catenin Stabilizes Adherens Junctions and Enhances DLC1 Antioncogenic Activity. Mol.Cell.Biol. 32:2145-2159 (#STA-403-A).
- Yu, X. et al. (2012). RhoGDI SUMOylation at Lys-138 Increases Its Binding Activity to Rho GTPase and Its Inhibiting Cancer Cell Motility. J.Biol. Chem. 287:13752-13760 (#STA-405).
- Geryk-Hall, M. et al. (2010). Driven to Death: Inhibition of Farnesylation Increases Ras Activity in Osteosarcoma and Promotes Growth Arrest and Cell Death. Mol. Cancer Ther. 9:1111-119 (#STA-400).
- Camalier, C.E. et al. (2010). Elevated Phosphate Activates N-ras and Promotes Cell Transformation and Skin Tumorigenesis. Cancer Prev. Res. 3:359-370 (#STA-400).
- Harmon, B. et al. (2008). Induction of the G-alpha-q Signaling Cascade by the Human Immunodeficiency Virus Envelope is Required for Virus Entry. J. Virol. 82:9191-9205 (#STA-400).
- Richier, L. et al. (2010). NOS1AP Associates with Scribble and Regulates Dendritic Spine Development. J. Neurosci. 30:4796-4805 (#STA-401-1).
- Takano, A. et al. (2009). Identification of Cectin-4 Oncoprotein as a Diagnostic and Therapeutic Target for Lung Cancer. Cancer Res. 69:6694-6703 (#STA-401-1).
- Shen, E. et al. (2009). Rac1 is Required for Cardiomyocyte Apoptosis During Hyperglycemia. Diabetes 58:2386-2395 (#STA-401-1).
- Lise, M-F. et al. (2009). Myosin-Va-Interacting Protein, RILPL2, Controls Cell Shape and Neuronal Morphogenesis via Rac Signaling. J. Cell Sci. 122:3810-3821 (#STA-401-1).
- Biname, F. et al. (2008). Transforming Growth Factor ß Controls the Directional Migration of Hepatocyte Cohorts by Modulating their Adhesion to Fibronectin. Mol. Biol. Cell 19:945-956 (#STA-401-1)
- Zhan, L. et al. (2008). Deregulation of Scribble Promotes Mammary Tumorigenesis and Reveals a Role for Cell Polarity in Carcinoma. Cell 135(5):865-878 (#STA-401-1).
- Leung, W. et al. (2007). The Inositol 5'-Phosphatase SHIP-2 Negatively Regulates IgE-Induced Mast Cell Degranulation and Cytokine Production. J. Immunol. 179:95-102 (#STA-401-1).
- Sturrock, A. et al. (2007). Nox4 mediates TGF-b1-Induced Retinoblastoma Protein Phosphorylation,Proliferation and Hypertrophy in Human Airway Smooth Muscle Cells. Am. J. Physiol Lung Cell Mol Physiol 292(6):L1543-55 (#STA-401-1).
- Fan, T-C. et al. (2007). A Heparan Sulfate-Facilitated and Raft-Dependent Macropinocytosis of Eosinophil Cationic Protein. Traffic 8(12):1778-1795 (#STA-401-1).
- Somervaille, T. et al. (2006). Identification and Characterization of Leukemia Stem Cells in Murine MLL-AF9 Acute Myeloid Leukemia. Cancer Cell 10:257-268 (#STA-401-1, STA-402).
- Zhang, S. et al. (2008). The Tumor Suppressor LKB1 Regulates Lung Cancer Cell Polarity by Mediating Cdc42 Recruitment and Activity. Cancer Res. 68:740-748 (#STA-402).
- Chen, H. et al. (2010). Integrity of SOS1/EPS8/ABI1 Tri-Complex Determines Ovarian Cancer Metastasis. Cancer Res. 70:9979-9990 (#STA-403-A, STA-404).
- Hayes, N.V.L. et al. (2010). Expression of Neuregulin 4 Splice Variants in Normal Human Tissues and Prostate Cancer and their Effects on Cell Motility. Endocr. Relat. Cancer. 18:39-49 (#STA-403-A)
- Godin, C.M. et al. (2010). The Angiotensin II Type 1 Receptor Induces Membrane Blebbing by Coupling to Rho A, Rho Kinase, and Myosin Light Chain Kinase. Mol.Pharmacol. 77:903-911. (#STA-403-A)
- Holmes,K.M.et al.(2012).Insulin-Like Growth Factor-Binding Protein 2-Driven Glioma Progression is Prevented by Blocking a Clinically Significant Integrin, Integrin-Linked Kinase, and NF-B Network.Proc Natl Acad Sci 109:2168-2173(#STA-404).
- Sultana, H. et al. (2010). Anaplasma Phagocytophilum Induces Actin Phosphorylation to Selectively Regulate Gene Transcription in Lxodes Scapularis Ticks. J. Exp. Med. 10.1084/jem.20100276 (#STA-404).
- Pandey, D. et al. (2009). Unraveling a Novel Rac1-Mediated Signaling Pathway that Regulates Cofilin Dephosphorylation and Secretion in Thrombin Stimulated Platelets. Blood 114:415-424 (#STA-404).
- Lorger, M. et al. (2006). Regulation of Epithelial Wound Closure and Intercellular Adhesion by Interaction of AF6 with Actin Cytoskeleton. J. of Cell Science 119:3385-3398 (#STA-404).
- Baranwai, S. et al. (2011). Molecular Characterization of the Tumor-Suppressive Function of Nischarin in Breast Cancer. J. Natl Cancer Inst. 10.1093/jnci/djr350 (#STA-405).
- Tian, D. et al. (2010). Antagonistic Regulation of Actin Dynamics and Cell Motility by TRPC5 and TRPC6 Channels. Sci. Signal. 3:ra77 (#STA-405).
- Ma, T. el al. (2010). Viral G Protein-Coupled Receptor Up-Regulates Angiopoietin-like 4 Promoting Angiogenesis and Vascular Permeability in Kaposi's Aarcoma. PNAS. 104:14363-14368. (#STA-405).
- Xu, Y. et al. (2010). Neuropilin-2 Mediates VEGF-C-Induced Lymphatic Sprouting Together with VEGFR3. J. Cell Biol. 188:115130 (#STA-405).
- Mohseni, M. et al. (2008). The Headpiece Domain of Dematin Regulates Cell Shape, Motility, and Wound Healing by Modulating RhoA Activity. Mol. Cell. Biol. 28:4712-4718 (#STA-405).
- Liu, Y. et al. (2008). Ganglioside Depletion and EGF Responses of Human GM3 Synthase-Deficient Fibroblasts. Glycobiology 18:593-601 (#STA-405).
- Qu, Y. et al. (2008). Enhanced mMgration and CXCR4 Over-Expression in Fibroblasts with Telomerase Reconstitution. Mol. and Cell. Biochem. 313:45-52 (#STA-405).
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