For UV-induced DNA Damage ELISA Kits (STA-322, STA-323, and STA-322-C): Standards or unknown DNA samples are first heat denatured before adsorbed onto a 96-well DNA binding plate. The sample or standard are then probed with an anti-6-4PP or CPD antibody, followed by an HRP conjugated secondary antibody.
For Cellular UV-Induced DNA Damage ELISA Kits (STA-326, and STA-328): cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-6-4PP or anti-CPD antibody, followed by an HRP conjugated secondary antibody.
For Cellular UV-Induced DNA Damage Staining Kits (STA-327 and STA-329): cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-6-4PP or anti-CPD antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.
