Alpha-Ketoglutarate Assay Kits

Alpha-KG Assays
  • 96-well colorimetric or fluorometric detection formats
  • Detect concentrations as low as 4 µM (colorimetric) or 0.2 µM (fluorometric)
  • Suitable for serum, plasma, cell suspensions, and tissue homogenates
  • Alpha-ketoglutarate standard provided for quantitation of unknown samples
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Alpha-Ketoglutarate Assay Kit (Colorimetric)
Catalog Number
MET-5131
Size
100 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$385.00
Alpha-Ketoglutarate Assay Kit (Fluorometric)
Catalog Number
MET-5132
Size
100 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$385.00
Product Details

Alpha-ketoglutarate is produced by the oxidative decarboxylation of isocitrate and is also the keto acid product of the deamination of glutamate. It exerts its influence in the TCA cycle as a rate determining intermediary of cellular energy metabolism. It also acts as anitrogen transporter that facilitates amino acid synthesis, while also inhibiting protein degradation in muscles.

Our Alpha-Ketoglutarate Assay Kits provide a simple and convenient method to measure alpha-KG in a 96-well plate format. Alpha-KG is transaminated resulting in the production of pyruvate, which is then detected with either a colorimetric or fluorometric probe. Each kit provides sufficient reagents for 100 assays, including blanks, standards, and unknown samples.