Recombinant viruses are excellent tools for the introduction of genetic material into host cells. Each virus differs in how it infects cells and replicates:
- Adenoviruses can infect a variety of mammalian cell types with high efficiency. They remain epichromosal upon infection, so they are only suitable for transient expression.
- Adeno-Associated Virus (AAV) integrates into the host cell genome at a very specific site in one human chromosome; random insertions are very rare, making it less immunogenic than adenovirus or retrovirus.
- Retroviruses (e.g. MMLV) introduce genetic material into the genome of the host cell, allowing long-term stable expression. Retroviral titer is typically lower than that of adenovirus.
- Lentiviruses (e.g. HIV-1, FIV, SIV) are a sub-class of retroviruses that can be used for both transient and stable gene expression; they can infect both proliferating and non-proliferating cells.
We have developed a variety of tools to improve the results of your viral expression studies. Our systems will take you from start to finish:
- Viral Packaging Systems
- Cell Lines designed for superior viral expression
- Premade Gene-Specific Viruses and Plasmids
- Virus Concentration and Purification Kits
- Viral Titer/Quantitation Kits
- Viral Transduction Reagents