Replication Competent Adenovirus Assay

  • More accurate than traditional plaque-forming unit assays
  • Faster results: 2.5 days vs. 10 days
  • No agar overlay steps
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Rapid RCA Assay Kit
Catalog Number
VPK-111
Size
30 assays
Detection
Immunocytochemistry
Manual/Data Sheet Download
SDS Download
Price
$420.00
Rapid RCA Assay Kit
Catalog Number
VPK-111-5
Size
5 x 30 assays
Detection
Immunocytochemistry
Manual/Data Sheet Download
SDS Download
Price
$1,850.00
Product Details

Traditionally RCA (replication competent adenovirus) is measured in permissive cells by a plaque-forming unit (PFU) assay which takes 10-14 days. Our Rapid RCA Assay Kit uses an immunocytochemistry staining protocol that requires only a two day incubation.

Assay Principle for the Rapid RCA Assay.

Staining Results from the Rapid RCA Assay. Serial 10-fold dilutions of Ad5 (2 x 10^12 viral particles/mL) were used to infect A549 cells in a 6-well plate for 48 hours. Anti-Hexon immunostaining was performed according to the assay protocol.

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