Lentivirus Transduction

Lentivirus Transduction
  • Proprietary reagent cocktail forms supercomplex with lentivirus
  • Increases lentivirus transduction efficiency 2- to 6-fold compared to Polybrene®
  • Useful for infection of non-permissive cells, including many primary cells and stem cells

 

Frequently Asked Questions about this product

General FAQs about Viral Gene Delivery

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ViraDuctin™ Lentivirus Transduction Kit
Catalog Number
LTV-200
Size
40 Transductions
Detection
N/A
Manual/Data Sheet Download
SDS Download
Price
$260.00
ViraDuctin™ Lentivirus Transduction Kit
Catalog Number
LTV-201
Size
200 Transductions
Detection
N/A
Manual/Data Sheet Download
SDS Download
Price
$620.00
Product Details

Lentiviral expression has many advantages over other viruses, including the ability to infect both proliferating and non-proliferating cells. The efficiency of lentivirus infection of target cells, however, tends to be lower. Additives such as Polybrene® can increase transduction efficiencies, but even then only a small fraction of lentiviral vectors can trasduce many target cell lines.

Our ViraDuctin™ Lentivirus Transduction Kit provides superior lentiviral transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene®. This system is ideal for many primary cells as well as immobilized cells.

*Note: The number of transductions per kit is based on use of a 24-well plate. The kit may also be used with 96-well, 12-well or 6-well plates, as well as 60 mm or 100 mm dishes. Please see product manual for more details.

Transduction of 293AD and HT-1080 Cells. 293AD cells (#AD-100) and HT-1080 cells were each seeded at 50,000 cells/well in a 24-well plate overnight. Cells were infected with GFP lentivirus for 48 hours in the presence of no additive (left), Polybrene® (middle), or the ViraDuctin™ Lentivirus Transduction Kit (right).

Transduction Efficiencies in Various Cell Lines. NIH3T3 cells, HeLa cells, our own 293AD cells (#AD-100), and HT-1080 cells were each seeded at 50,000 cells/well in a 24-well plate overnight. Cells were infected with GFP lentivirus for 48 hours in the presence of Polybrene® or the ViraDuctin™ Lentivirus Transduction Kit. For each cell line, fluorescence levels using the ViraDuctin™ Lentivirus Kit are depicted relative to a normalized fluorescence level of 100 for transductions with Polybrene®.

Recent Product Citations
  1. Nakamura, M. et al. (2020). MicroRNA‑22 enhances radiosensitivity in cervical cancer cell lines via direct inhibition of c‑Myc binding protein, and the subsequent reduction in hTERT expression. Oncology Letters. 19:2213-2222. doi: 10.3892/ol.2020.11344.
  2. Bokelmann, M. et al. (2020). Utility of primary cells to examine NPC1 receptor expression in Mops condylurus, a potential Ebola virus reservoir. PLoS Negl Trop Dis. 14(1):e0007952. doi: 10.1371/journal.pntd.0007952.
  3. Huang, C. et al. (2019). EphA2-to-YAP Pathway Drives Gastric Cancer Growth and Therapy Resistance. Int J Cancer. doi: 10.1002/ijc.32609.
  4. Zhao, R.B. et al. (2018). High-throughput sequencing analysis of lncRNAs in hippocampus tissues with hypoxic-ischemic brain damage. Int J Clin Exp Pathol. 11(11):5265-5277.
  5. Kim, S.C. et al. (2018). Establishment and Characterization of Paired Primary and Peritoneal Seeding Human Colorectal Cancer Cell Lines: Identification of Genes That Mediate Metastatic Potential. Transl Oncol. 11(5):1232-1243. doi: 10.1016/j.tranon.2018.07.014.
  6. Kraniak, J.M. et al. (2018). Development of 3D culture models of plexiform neurofibroma and initial application for phenotypic characterization and drug screening. Experimental Neurology. 299:Part B: 289-298.
  7. Fang, X. et al. (2016). IKKα-mediated biogenesis of miR-196a through interaction with Drosha regulates the sensitivity of cancer cells to radiotherapy. Cell Death Differ. doi:10.1038/cdd.2016.32.
  8. Osorio, L. A. et al. (2015). SNAIL transcription factor increases the motility and invasive capacity of prostate cancer cells. Mol Med Rep. 13:778-786.
  9. Kandasamy, K. et al. (2015). Changes in endothelial Cx43 expression inversely correlates with microvessel permeability and VE-cadherin expression in endotoxin challenged lungs. Am J Physiol Lung Cell Mol Physiol. doi: 10.1152/ajplung.00211.2014.
  10. Abel, E. V. et al. (2014). The Notch pathway is important in maintaining the cancer stem cell population in pancreatic cancer. PLoS One. 9:e91983.
  11. Ozelo, M.C. et al. (2014).  Omental implantation of BOECs in hemophilia dogs results in circulating FVIII antigen and a complex immune response. Blood. 123:4045-4053.
  12. Rossello, R.A. et al. (2013). Mammalian Genes Induce Partially Reprogrammed Pluripotent Stem Cells in Non-Mammalian Vertebrate and Invertebrate Species. eLife Sci. 2:e00036 (#LTV-201).
  13. McEachron, T.A. et al. (2010). Protease-activated Receptors Mediate Crosstalk between Coagulation and Fibrinolysis. Blood 116:5037-5044. (#LTV-201)
  14. Zemskova, M. et al. (2010). p53-Dependent Induction of Prostate Cancer Cell Senescence by the PIM1 Protein Kinase. Mol. Cancer Res. 8:1126-1141. (#LTV-201)