Lentivirus-Associated p24 ELISA Kit

Lentivirus-Associated p24 ELISA Kit
  • Proprietary technology separates virus-associated p24 from free p24
  • Minimizes overestimation of lentivirus titer common to traditional p24 ELISA kits
  • Lentivirus quantitation on a standard microplate reader
  • HIV-1 p24 Standard included

 

Frequently Asked Questions about this product

General FAQs about Viral Gene Delivery

Video: Color Development in an ELISA

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QuickTiter™ Lentivirus Titer Kit (Lentivirus-Associated HIV p24)
Catalog Number
VPK-107-5
Size
5 x 96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$2,695.00
QuickTiter™ Lentivirus Titer Kit (Lentivirus-Associated HIV p24)
Catalog Number
VPK-107
Size
96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$635.00
QuickTiter™ Lentivirus Titer Kit (Lentivirus-Associated HIV p24), Trial Size
Catalog Number
VPK-107-T
Size
32 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$315.00
Product Details

Measuring the HIV-1 p24 antigen is a long-established method for lentivirus quantitation. However, the traditional p24 ELISA detects both virus-associated p24 and free p24 generated by 293 cells during transient transfection. Free p24 can account for a substantial portion of total p24 in the supernatant. Therefore, the ELISA typically overestimates the quantity of lentivirus present.

Our QuickTiter™ Lentivirus Titer Kit (Lentivirus-Associated HIV p24) substantially minimizes this problem. A proprietary technology separates the lentivirus from free p24 in solution prior to running the ELISA portion of the assay.

Assay Principle for the QuickTiter Lentivirus Titer Kit (Lentivirus-Associated p24 ELISA).

ViraBind™ Lentivirus Reagents Successfully Separate Free p24 from Lentivirus-Associated p24. Recombinant p24 diluted in culture medium was treated with ViraBind™ Lentivirus Reagents. The amount of p24 in the supernatant and pellet was measured according to the assay protocol.

Recent Product Citations
  1. Fernandes-Junior, S.A. et al. (2020). Stimulation of Retrotrapezoid Nucleus Phox2b-expressing Neurons Rescues Breathing Dysfunction in an Experimental Parkinson's Disease Rat Model. Brain Pathol. doi: 10.1111/bpa.12868.
  2. Folegatti, P.M. et al. (2020). Safety and immunogenicity of a candidate Middle East respiratory syndrome coronavirus viral-vectored vaccine: a dose-escalation, open-label, non-randomised, uncontrolled, phase 1 trial. Lancet Infect Dis. pii: S1473-3099(20)30160-2. doi: 10.1016/S1473-3099(20)30160-2.
  3. Purroy, R. et al. (2020). Frataxin-deficient cardiomyocytes present an altered thiol-redox state which targets actin and pyruvate dehydrogenase. Redox Biology. 32:101520. doi: 10.1016/j.redox.2020.101520.
  4. Javidi-Parsijani, P. et al. (2020). CRISPR/Cas9 increases mitotic gene conversion in human cells. Gene Ther. doi: 10.1038/s41434-020-0126-z.
  5. Yang, H. et al. (2020). Understanding the structural basis of HIV-1 restriction by the full length double-domain APOBEC3G. Nat Commun. 11(1):632. doi: 10.1038/s41467-020-14377-y.
  6. Goo, J. et al. (2020). Characterization of novel monoclonal antibodies against MERS-coronavirus spike protein. Virus Res. doi: 10.1016/j.virusres.2020.197863.
  7. Czarnek, M. et al. (2019). Proteolytic Processing of Neuregulin 2. Mol Neurobiol. doi: 10.1007/s12035-019-01846-9.
  8. Pan, X. et al. (2019). Lysine-specific demethylase-1 regulates fibroblast activation in pulmonary fibrosis via TGF-β1/Smad3 pathway. Pharmacol Res. 152:104592. doi: 10.1016/j.phrs.2019.104592.
  9. Bussiere, R. et al. (2019). Upregulation of the Sarco-Endoplasmic Reticulum Calcium ATPase 1 Truncated Isoform Plays a Pathogenic Role in Alzheimer's Disease. Cells. 8(12). pii: E1539. doi: 10.3390/cells8121539.
  10. Jha, A. et al. (2019). Alterations in Plasma Membrane Ion Channel Structures Stimulate NLRP3 Inflammasomes Activation in APOL1 Risk Milieu. FEBS J. doi: 10.1111/febs.15133.
  11. Zhang, S. et al. (2019). The resistance of esophageal cancer cells to paclitaxel can be reduced by the knockdown of long noncoding RNA DDX11-AS1 through TAF1/TOP2A inhibition. Am J Cancer Res. 9(10):2233-2248.
  12. Park, T.Y. et al. (2019). Chloroquine modulates inflammatory autoimmune responses through Nurr1 in autoimmune diseases. Sci Rep. 9(1):15559. doi: 10.1038/s41598-019-52085-w.
  13. Gonzalez, S.M. et al. (2019). Vitamin D treatment of peripheral blood mononuclear cells modulated immune activation and reduced susceptibility to HIV-1 infection of CD4+ T lymphocytes. PLoS One. 14(9):e0222878. doi: 10.1371/journal.pone.0222878.
  14. Ruscic, J. et al. (2019). Lentiviral Vector Purification Using Nanofiber Ion Exchange Chromatography. Mol Ther Methods Clin Dev. doi: 10.1016/j.omtm.2019.08.007.
  15. Poorebrahim, M. et al. (2019). Production of CAR T-cells by GMP-grade lentiviral vectors: Latest advances and future prospects. Crit Rev Clin Lab Sci. doi: 10.1080/10408363.2019.1633512.
  16. Blomberg, R. et al. (2019). Fibroblast activation protein restrains adipogenic differentiation and regulates matrix-mediated mTOR signaling. Matrix Biol. pii: S0945-053X(19)30189-1. doi: 10.1016/j.matbio.2019.07.007.
  17. Lyu, P. et al. (2019). Delivering Cas9/sgRNA ribonucleoprotein (RNP) by lentiviral capsid-based bionanoparticles for efficient 'hit-and-run' genome editing. Nucleic Acids Res. pii: gkz605. doi: 10.1093/nar/gkz605.
  18. Ommer, A. et al. (2019). Ral GTPases in Schwann cells promote radial axonal sorting in the peripheral nervous system. J Cell Biol. pii: jcb.201811150. doi: 10.1083/jcb.201811150.
  19. Kumar, R. et al. (2019). The Cytosine Deaminase AICDA Regulates FGF/ERK Signaling to Achieve the Naïve Pluripotent State During Reprogramming. Stem Cells. doi: 10.1002/stem.3023.
  20. Zhang, M. et al. (2019). Downregulation of miR143/145 gene cluster expression promotes the aortic media degeneration process via the TGF-β1 signaling pathway. Am J Transl Res. 11(1):370-378.
  21. Lee, S.Y. et al. (2019). Optogenetic control of iPS cell-derived neurons in 2D and 3D culture systems using channelrhodopsin-2 expression driven by the synapsin-1 and calcium-calmodulin kinase II promoters. J Tissue Eng Regen Med. 13(3):369-384. doi: 10.1002/term.2786.
  22. DaSilva-Arnold, S.C. et al. (2019). ZEB2, a master regulator of the epithelial-mesenchymal transition, mediates trophoblast differentiation. Mol Hum Reprod. 25(2):61-75. doi: 10.1093/molehr/gay053.
  23. Mohammadzadeh, N. et al. (2019). Polymorphisms of the cytidine deaminase APOBEC3F have different HIV-1 restriction efficiencies. Virology. 527:21-31. doi: 10.1016/j.virol.2018.11.004.
  24. Xie, X. et al. (2019). PPPDE1 promotes hepatocellular carcinoma development by negatively regulate p53 and apoptosis. Apoptosis. 24(1-2):135-144. doi: 10.1007/s10495-018-1491-6.
  25. Sanz, R. et al. (2019). In Situ Peroxidase Labeling and Mass Spectrometry of Alpha-Synuclein in Rat Cortical Neurons. Methods Mol Biol. 1948:235-246. doi: 10.1007/978-1-4939-9124-2_18.
  26. Lu, B. et al. (2019). Delivering SaCas9 mRNA by lentivirus-like bionanoparticles for transient expression and efficient genome editing. Nucleic Acids Res. doi: 10.1093/nar/gkz093.
  27. Varela, M. et al. (2019). Extracellular mycobacterial DNA drives disease progression by triggering Caspase-11-dependent pyroptosis of infected macrophages. bioRxiv. 514125. doi: 10.1101/514125.
  28. Fanning, S. et al. (2019). Lipidomic Analysis of α-Synuclein Neurotoxicity Identifies Stearoyl CoA Desaturase as a Target for Parkinson Treatment. Mol Cell. 73(5):1001-1014.e8. doi: 10.1016/j.molcel.2018.11.028.
  29. Malheiros-Lima, M.R. et al. (2018). Breathing responses produced by optogenetic stimulation of adrenergic C1 neurons are dependent on the connection with preBötzinger complex in rats. Pflugers Arch - Eur J Physiol. 470: 1659. doi: 10.1007/s00424-018-2186-0.
  30. George, J.H. et al. (2018). A closer look at neuron interaction with track-etched microporous membranes. Sci Rep. 8(1):15552. doi: 10.1038/s41598-018-33710-6.