Lentivirus Transduction

Lentivirus Transduction
  • Proprietary reagent cocktail forms supercomplex with lentivirus
  • Increases lentivirus transduction efficiency 2- to 6-fold compared to Polybrene®
  • Useful for infection of non-permissive cells, including many primary cells and stem cells

 

Frequently Asked Questions about this product

General FAQs about Viral Gene Delivery

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ViraDuctin™ Lentivirus Transduction Kit
Catalog Number
LTV-200
Size
40 Transductions
Detection
N/A
Manual/Data Sheet Download
SDS Download
Price
$275.00
ViraDuctin™ Lentivirus Transduction Kit
Catalog Number
LTV-201
Size
200 Transductions
Detection
N/A
Manual/Data Sheet Download
SDS Download
Price
$660.00
Product Details

Lentiviral expression has many advantages over other viruses, including the ability to infect both proliferating and non-proliferating cells. The efficiency of lentivirus infection of target cells, however, tends to be lower. Additives such as Polybrene® can increase transduction efficiencies, but even then only a small fraction of lentiviral vectors can trasduce many target cell lines.

Our ViraDuctin™ Lentivirus Transduction Kit provides superior lentiviral transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene®. This system is ideal for many primary cells as well as immobilized cells.

*Note: The number of transductions per kit is based on use of a 24-well plate. The kit may also be used with 96-well, 12-well or 6-well plates, as well as 60 mm or 100 mm dishes. Please see product manual for more details.

Transduction of 293AD and HT-1080 Cells. 293AD cells (#AD-100) and HT-1080 cells were each seeded at 50,000 cells/well in a 24-well plate overnight. Cells were infected with GFP lentivirus for 48 hours in the presence of no additive (left), Polybrene® (middle), or the ViraDuctin™ Lentivirus Transduction Kit (right).

Transduction Efficiencies in Various Cell Lines. NIH3T3 cells, HeLa cells, our own 293AD cells (#AD-100), and HT-1080 cells were each seeded at 50,000 cells/well in a 24-well plate overnight. Cells were infected with GFP lentivirus for 48 hours in the presence of Polybrene® or the ViraDuctin™ Lentivirus Transduction Kit. For each cell line, fluorescence levels using the ViraDuctin™ Lentivirus Kit are depicted relative to a normalized fluorescence level of 100 for transductions with Polybrene®.

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