Retrovirus Transduction

The efficiency of retrovirus infection of target cells tends to be lower than that of adenovirus. Additives such as Polybrene® can increase transduction efficiencies, but even then only a small fraction of retroviral vectors can trasduce many target cell lines.

Our ViraDuctin™ Retrovirus Transduction Kit provides superior transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene®. This system is ideal for many primary cells as well as immobilized cells.

*Note: The number of transductions per kit is based on use of a 24-well plate. The kit may also be used with 96-well, 12-well or 6-well plates, as well as 60 mm or 100 mm dishes. Please see product manual for more details.

1. Liu, D. et al. (2016). Retrogenic ICOS expression increases differentiation of KLRG-1hiCD127loCD8+ T cells during listeria infection and diminishes recall responses. J Immunol. 196:1000-1012.
2. Gandhi, M. et al. (2012). Homologous Chromosomes Make Contact at the Sites of Double-Strand Breaks in Genes in Somatic G0/G1-Phase Human Cells. PNAS. 109:9454-9459.
3. Miyoshi, N. et al. (2010). Defined factors induce reprogramming of gastrointestinal cancer cells. PNAS 107:40-45.