If you are assaying both invasive and migratory properties of your cells, order one of our economic CytoSelect™ Cell Migration / Invasion Assay Combo kits. These kits save you money compared to buying separate chemotaxis and cell invasion kits.
Each 24-well combo kit provides sufficient reagents to perform 12 cell migration plus 12 cell invasion assays.
Figure 1: Migration of Human Fibrosarcoma HT-1080 Cells.Cells were seeded at 30,000 cells per well of a 24-well plate and allowed to migrate toward 10% FBS for 4 hours in either the presence or absence of 2µM Cytochalasin D. Migratory cells on the bottom of the polycarbonate membrane were stained (top) or quantified in a fluorescence plate reader (bottom).
Figure 2: Effects of Cytochalasin D on Invading Cells using the CytoSelect™ 24-Well Cell Invasion Assay. HT-1080 and NIH3T3 cells (negative control) were seeded at 300,000 cells/well and allowed to invade toward 10% FBS for 24 hrs in the presence or absence of 2µM Cytochalasin D. Invasive cells, on the bottom of the invasion membrane, were stained (above) and then quantified at OD 560 nm after extraction using a standard plate reader (not shown).