Cell Transformation / Soft Agar Colony Assays
Transformation of normal cells into neoplastic cells occurs via a series of genetic and epigenetic alterations, yielding a cell population capable of proliferation independently of both internal and external signals that normally restrain their growth. Traditionally the soft agar colony formation assay has been used to monitor cell transformation and anchorage-independent growth, with manual counting of proliferated cells after 3-4 weeks of cell growth.
Cell Biolabs has improved the traditional soft agar assay with the following benefits:
- Eliminate tedious manual colony counting under a microscope
- Obtain results in a fraction of the time
- Higher-throughput format
- More accurate results
- Special Cell Recovery format allows recovery of viable intact cells for further analysis
We offer soft agar colony assays in a variety of formats:
- Our 96-Well Standard Soft Agar Assay combines a soft agar medium with a fluorescent dye to eliminate manual cell counting and provide faster results. This kit is ideal if cells do not need to be recovered.
- Our 96-Well Soft Agar Assay with Cell Recovery advances the technology even further. This kit uses a proprietary soft agar-based medium that allows transformed cells to be recovered after culture for further downstream analysis.
- Our 96-Well In Vitro Tumor Sensitivity Assay provides a stringent, anchorage-independent model for chemosenstivity testing and screening of potential anticancer drugs.