Transformation of normal cells into neoplastic cells occurs via a series of genetic and epigenetic alterations, yielding a cell population capable of proliferation independently of both internal and external signals that normally restrain their growth. Traditionally the soft agar colony formation assay has been used to monitor cell transformation and anchorage-independent growth, with manual counting of proliferated cells after 3-4 weeks of cell growth.
Cell Biolabs has improved the traditional soft agar assay to provide increased throughput and more accurate results. Assay times are reduced to about one week, and tedious manual cell counting has been eliminated. We offer soft agar colony assays in a variety of formats: