24-Well Haptotaxis Assays, Collagen I

24-Well Haptotaxis Assays, Collagen I
  • Fully quantify haptotaxis with no manual cell counting
  • Measure haptotaxis in less than 6 hours with most cell types
  • Membrane inserts are precoated on the bottom with Collagen I
  • Colorimetric or fluorometric detection

 

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CytoSelect™ 24-Well Cell Haptotaxis Assay, Collagen I
Catalog Number
CBA-100-COL
Size
12 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$465.00
CytoSelect™ 24-Well Cell Haptotaxis Assay, Collagen I
Catalog Number
CBA-101-COL
Size
12 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$490.00
Product Details

Cell haptotaxis describes cell migration toward or along a gradient of chemoattractants or adhesion sites in the extracellular matrix.

The CytoSelect™ Cell Haptotaxis Assays are ideal for determining the migratory properties of cells. The 8 µm membrane pore size is ideal for epithelial cells and fibroblasts. The membrane serves as a barrier to distinguish between migratory and non-migratory cells.

CytoSelect™ 24-Well Cell Haptotaxis Assay. MDA-231 cells were seeded at 150,000 cells/well and allowed to migrate toward FBS for 4 hours. Migratory cells, on the bottom of the migration membrane, were stained according to the assay protocol.

Recent Product Citations
  1. Orlandella, F.M. et al. (2019). miR-650 promotes motility of anaplastic thyroid cancer cells by targeting PPP2CA. Endocrine. doi: 10.1007/s12020-019-01910-3 (#CBA-100-COL).
  2. Xu, Y. et al. (2018). Sipa1 deficiency unleashes a host-immune mechanism eradicating chronic myelogenous leukemia-initiating cells. Nat Commun. 9(1):914. doi: 10.1038/s41467-018-03307-8 (#CBA-100-COL).
  3. Bezhaeva, T. et al. (2018). Relaxin receptor deficiency promotes vascular inflammation and impairs outward remodeling in arteriovenous fistulas. FASEB J. fj201800437R. doi: 10.1096/fj.201800437R (#CBA-101-COL).
  4. Singh, D.R. et al. (2016). The SAM domain inhibits EphA2 interactions in the plasma membrane. Biochim Biophys. Acta doi:10.1016/j.bbamcr.2016.10.011 (#CBA-101-COL).
  5. Singh, D.R. et al. (2015). EphA2 unliganded dimers suppress EphA2 pro-tumorigenic signaling. J Biol Chem. doi:10.1074/jbc.M115.676866 (#CBA-101-COL).
  6. Yamauchi, A. et al. (2017). Evaluation of pancreatic cancer cell migration with multiple parameters in vitro by using an optical real-time cell mobility assay device. BMC Cancer. 17(1):234. doi: 10.1186/s12885-017-3218-4 (#CBA-100-COL).
  7. Herrera, I. et al. (2013). Matrix Metalloproteinase (MMP)-1 Induces Lung Alveolar Epithelial Cell Migration and Proliferation, Protects from Apoptosis, and Represses Mitochondrial Oxygen Consumption. J. Biol. Chem. 288:25964-25975 (#CBA-100-COL).
  8. Niccoli, S. et al. (2012).The Asian-American E6 Variant Protein of Human Papillomavirus 16 Alone Is Sufficient To Promote Immortalization, Transformation, and Migration of Primary Human Foreskin Keratinocytes. J Virol. 86:12384-12396 (#CBA-100-COL).
  9. Kamiya, K. et al. (2007). Protein Kinase C Delta Activated Adhesion Regulates Vascular Smooth Muscle Cell Migration. J. Surg. Res. 141:91-96. (#CBA-100-COL).