Tryptophan Assay Kit

Tryptophan Assay Kit
  • Detection sensitivity limit of approximately 1.6 µM Tryptophan
  • Suitable for use with lysates, serum, plasma, urine and culture supernatants
  • L-Tryptophan standard included

NOTE: Each sample replicate requires 2 assays for proper data calculation.

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Tryptophan Assay Kit
Catalog Number
100 assays
Manual/Data Sheet Download
SDS Download
Product Details

The Tryptophan Assay Kit is a sensitive quantitative fluorometric assay for tryptophan. Tryptophan Oxidase converts tryptophan to 2-imino-3(indol-3-yl) propanoate and hydrogen peroxide (H2O2). The H2O2 is then detected with a highly specific fluorometric probe.  Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples and standards are incubated for 60 minutes and then read with a standard 96-well fluorometric plate reader. Samples are compared to a known concentration of tryptophan standard within the 96-well microtiter plate format.