Cell Biolabs’ PQQ Assay Kit is a convenient quantitative tool that measures PQQ within biological samples. The assay is based on an enzymatic activity reaction of glucose dehydrogenase (GDH). E. coli GDH lacks activity unless PQQ is present. Active GDH results in an electron transfer to a colorimetric probe that produces a colored product that can be measured at 450 nm. The intensity of the product color is proportional to the amount of PQQ within a sample. Samples are compared to a known concentration of PQQ standard within the 96-well microtiter plate format.
