Alkaline Phosphatase Activity Assays, 96-Well

Alkaline Phosphatase Activity Assays, 96-Well
  • Measure the ubiquitous alkaline phosphatase marker in embryonic stem cells and embryonic germ cells
  • Quantify activity by colorimetric or fluorometric plate reader


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StemTAG™ Alkaline Phosphatase Activity Assay Kit
Catalog Number
100 assays
Manual/Data Sheet Download
SDS Download
Product Details

Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG™ Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker.

The StemTAG™ Alkaline Phosphatase Activity Assay Kits provide reagents for quantifying alkaline phosphatase activity in a convenient 96-well plate format, with either colorimetric or fluorescence detection.

Staining of Undifferentiated and Differentiated ES cells using the StemTAG™ Alkaline Phosphatase Staining Kit. Murine embryonic stem cells (ES-D3) were maintained in an undifferentiated state on gelatin-coated dishes in the presence of LIF. To induce differentiation, LIF was withdrawn over a period of several days; various differentiation events were observed (cells became flattened and enlarged with reduced proliferation). At the end of day 5, cells were stained according to the StemTAG™ Alkaline Phosphatase Staining Kit assay protocol.

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