Cell Biolabs’ ATP Assay Kit is a simple fluorometric assay that measures the amount of total ATP present in biological samples in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays*, including blanks, ATP standards, and unknown samples. Sample ATP concentrations are determined by comparison with a known ATP standard. The kit has a detection sensitivity limit of 1.56 µM ATP.
*Note: Each sample replicate requires 2 assays, one treated with Glycerol Kinase (+GK) and one without (-GK). ATP is calculated from the difference in RFU readings from the 2 wells.