Branched Chain Amino Acid Assay Kit

Branched Chain Amino Acid Assay Kit
  • Measures only free branched chain amino acids; does not detect peptides or proteins
  • Detection sensitivity limit of 15.6 µM
  • Suitable for use with lysates, serum, plasma, urine, and food samples
  • L-Leucine standard included
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Branched Chain Amino Acid Assay Kit
Catalog Number
MET-5056
Size
192 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$375.00
Product Details

Branched chain amino acids (BCAA) contain a branch structure side chain with one central carbon connected to at least 3 other carbon atoms. BCAAs in humans include leucine, isoleucine, and valine. BCAAs play key roles in metabolism and the immune system, and blood levels have been shown to be elevated in obese, insulin-resistant rat and mouse models.

Our Branched Amino Acid Assay Kit is a simple colorimetric assay that measures the total amount of all free BCAAs. The branched chain amino acids are converted by Leucine Dehydrogenase into alpha-keto products plus ammonia and NADH. The NADH is then detected with WST-1 which is converted into a formazan form that can be detected by a 96-well colorimetric plate reader.. BCAA levels in an unknown sample are calculated based on a known L-Leucine standard curve.

Recent Product Citations
  1. Sing, C.N. et al. (2022). Identification of a modulator of the actin cytoskeleton, mitochondria, nutrient metabolism and lifespan in yeast. Nat Commun. 13(1):2706. doi: 10.1038/s41467-022-30045-9.
  2. Akasu, R. et al. (2022). Calpain-mediated proteolytic production of free amino acids in vascular endothelial cells augments obesity-induced hepatic steatosis. J Biol Chem. doi: 10.1016/j.jbc.2022.101953.