The Cas9 protein (CRISPR associated protein 9) has gained attention as a genome engineering tool to cause site-directed double strand breaks in DNA. Resulting DNA breaks can inactivate genes or introduce heterologous genes through non-homologous end joining and homologous recombination, respectively, in many model organisms. Both gene deletion and gene replacement have been demonstrated using the CRISPR/Cas9 system in human cells.
Our Cas9 ELISA Kit is an immunoassay developed for the detection and quantitation of S. pyogenes Cas9 in lysates made from cells or tissues. Unknown samples are compared against a Cas9 standard curve. Each kit provides sufficient reagents for up to 96 assays including standards and unknown samples.