96-Well Cellular Senescence Activity Assay

96-Well Cellular Senescence Activity Assay
  • Measure activity of senescence-associated ß-galactosidase
  • Quantitative results in a fluorescence plate reader

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96-Well Cellular Senescence Assay (SA β-Gal Activity)
Catalog Number
CBA-231
Size
120 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$585.00
96-Well Cellular Senescence Assay Kit (SA β-Gal Activity)
Catalog Number
CBA-231-5
Size
5 x 120 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$2,590.00
Product Details

Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader.

SA-ß-Gal activity in Senescent Human Lung Fibroblast HFL-1 Cells. Normal HFL-1 cells with different passage numbers were lysed. Lysates were allowed to incubate with SA-ß-Gal Substrate for 1 hr at 37ºC.

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  1. Tsutsui, Y. et al. (2026). Human iPSC-based Modeling of Pulmonary Fibrosis Reveals p300/CBP Inhibition Suppresses Alveolar Transitional Cell State. Nat Commun. 17(1):1214. doi: 10.1038/s41467-026-68909-z.
  2. Zhou, Y. et al. (2025). Antioxidant and Anti-Senescence Polyvinyl Alcohol-Gallic Acid Supramolecular Hydrogels for Stem Cell Culture. Adv Healthc Mater. doi: 10.1002/adhm.202402882.
  3. Tarasova, K. et al. (2025). Dexamethasone: a double-edged sword in the treatment of osteoarthritis. Sci Rep. 15(1):11832. doi: 10.1038/s41598-025-96050-2.
  4. Dzubanova, M. et al. (2025). Protocol for isolation of human bone marrow stromal cells and characterization of cellular metabolism. STAR Protoc. 6(1):103553. doi: 10.1016/j.xpro.2024.103553.
  5. Haroon, M. & Kang, S.C. (2024). Kaempferol Synergistically Enhances Cisplatin-induced Apoptosis and Cell Cycle Arrest in Colon Cancer Cells. J Cancer Prev. 29(3):69-87. doi: 10.15430/JCP.24.013.
  6. Namirah, I. et al. (2024). The effect of ethanol-based coriander (Coriandrum sativum L.) seed extract on oxidative stress, antioxidant level and cellular senescence in the heart of obese rat. J Pharm Pharmacogn Res. 12(6):1111-1120. doi: 10.56499/jppres24.1957_12.6.1111.
  7. Zhang, L. et al. (2024). Nesfatin-1 attenuated lipopolysaccharide-induced inflammatory response and senescence in human dental pulp cells. Heliyon. 10(12):e32108. doi: 10.1016/j.heliyon.2024.e32108.
  8. Boulestreau, J. et al. (2024). Extracellular vesicles from senescent mesenchymal stromal cells are defective and cannot prevent osteoarthritis. J Nanobiotechnology. 22(1):255. doi: 10.1186/s12951-024-02509-1.
  9. Oram, M.K. et al. (2024). RNF4 prevents genomic instability caused by chronic DNA under-replication. DNA Repair (Amst). doi: 10.1016/j.dnarep.2024.103646.
  10. Hardiany, N.S. et al. (2024). Exploration of neuroprotective effect from Coriandrum sativum L. ethanolic seeds extracts on brain of obese rats. Sci Rep. 14(1):603. doi: 10.1038/s41598-024-51221-5.
  11. Bai, W. et al. (2024). S-nitrosylation of AMPKγ impairs coronary collateral circulation and disrupts VSMC reprogramming. EMBO Rep. 25(1):128-143. doi: 10.1038/s44319-023-00015-3.
  12. Benova, A. et al. (2023). Omega-3 PUFAs prevent bone impairment and bone marrow adiposity in mouse model of obesity. Commun Biol. 6(1):1043. doi: 10.1038/s42003-023-05407-8.
  13. Shen, C.Y. et al. (2023). Unveiling the molecular basis of inflamm-aging induced by advanced glycation end products (AGEs)-modified human serum albumin (AGE-HSA) in patients with different immune-mediated diseases. Clin Immunol. 252:109655. doi: 10.1016/j.clim.2023.109655.
  14. Ambrosio, M.R. et al. (2023). Targeting G-quadruplex motifs interferes with differentiation of adipose-derived mesenchymal stem cells. Stem Cell Res Ther. 14(1):98. doi: 10.1186/s13287-023-03320-9.
  15. Choi, D. et al. (2023). Vertical Vibration of Mouse Osteoblasts Promotes Cellular Differentiation and Cell Cycle Progression and Induces Aging In Vitro. Biomedicines. 11(2):444. doi: 10.3390/biomedicines11020444.
  16. Takami, Y. et al. (2022). Novel pathophysiological roles of α-synuclein in age-related vascular endothelial dysfunction. FASEB J. 36(10):e22555. doi: 10.1096/fj.202101621R.
  17. Nelczyk, A.T. et al. (2022). The nuclear receptor TLX (NR2E1) inhibits growth and progression of triple- negative breast cancer. Biochim Biophys Acta Mol Basis Dis. 1868(11):166515. doi: 10.1016/j.bbadis.2022.166515.
  18. Chelyapov, N. et al. (2022). Autologous NK cells propagated and activated ex vivo decrease senescence markers in human PBMCs. Biochem Biophys Rep. doi: 10.1016/j.bbrep.2022.101380.
  19. Furukawa, M. et al. (2022). Analysis of senescence in gingival tissues and gingival fibroblast cultures. Clin Exp Dent Res. doi: 10.1002/cre2.581.
  20. Kelleher, A.M. et al. (2021). Deficiency of PARP-1 and PARP-2 in the mouse uterus results in decidualization failure and pregnancy loss. Proc Natl Acad Sci U S A. 118(40):e2109252118. doi: 10.1073/pnas.2109252118.
  21. Deng, Z. et al. (2021). Biofunction of Polydopamine Coating in Stem Cell Culture. ACS Appl Mater Interfaces. doi: 10.1021/acsami.0c22565.
  22. Lee, Y.N. et al. (2021). Ultrasonic microbubble VEGF gene delivery improves angiogenesis of senescent endothelial progenitor cells. Sci Rep. 11(1):13449. doi: 10.1038/s41598-021-92754-3.
  23. Hirata, Y. et al. (2021). Advanced maternal age induces fetal growth restriction through decreased placental inflammatory cytokine expression and immune cell accumulation in mice. J Reprod Dev. doi: 10.1262/jrd.2021-034.
  24. Mehdi, S.J. et al. (2021). Normal and cancer fibroblasts differentially regulate TWIST1, TOX and cytokine gene expression in cutaneous T-cell lymphoma. BMC Cancer. 21(1):492. doi: 10.1186/s12885-021-08142-7.
  25. Bourdon, B. et al. (2021). Marine Collagen Hydrolysates Promote Collagen Synthesis, Viability and Proliferation While Downregulating the Synthesis of Pro-Catabolic Markers in Human Articular Chondrocytes. Int. J. Mol. Sci. 22(7):3693. doi: 10.3390/ijms22073693.
  26. Kong, C.S. et al. (2021). Embryo biosensing by uterine natural killer cells determines endometrial fate decisions at implantation. FASEB J. 35(4):e21336. doi: 10.1096/fj.202002217R.
  27. Baxley, R.M. et al. (2021). Bi-allelic MCM10 variants associated with immune dysfunction and cardiomyopathy cause telomere shortening. Nat Commun. 12(1):1626. doi: 10.1038/s41467-021-21878-x.
  28. Yamaguchi, S. et al. (2021). Characterization of an active LINE-1 in the naked mole-rat genome. Sci Rep. 11(1):5725. doi: 10.1038/s41598-021-84962-8.
  29. Bourdon, B. et al. (2021). Marine Collagen Hydrolysates Downregulate the Synthesis of Pro-Catabolic and Pro-Inflammatory Markers of Osteoarthritis and Favor Collagen Production and Metabolic Activity in Equine Articular Chondrocyte Organoids. Int J Mol Sci. 22(2):E580. doi: 10.3390/ijms22020580.
  30. Jiang, Y. et al. (2021). Histone H3K27 methyltransferase EZH2 and demethylase JMJD3 regulate hepatic stellate cells activation and liver fibrosis. Theranostics. 11(1):361-378. doi: 10.7150/thno.46360.