CML Competitive ELISA

  • Detect CML as low as 2.25 ng/mL from a variety of samples
  • CML-modified BSA included as standard
  • Compatible with cell lysates, serum, plasma, purified proteins, and other protein-containing samples

 

Frequently Asked Questions about this product

General FAQs about Oxidative Stress

Video: Color Development in an ELISA

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OxiSelect™ N-epsilon-(Carboxymethyl) Lysine (CML) Competitive ELISA Kit
Catalog Number
STA-816
Size
96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$645.00
OxiSelect™ N-epsilon-(Carboxymethyl) Lysine (CML) Competitive ELISA Kit
Catalog Number
STA-816-5
Size
5 x 96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$2,750.00
OxiSelect™ N-epsilon-(Carboxymethyl) Lysine (CML) Competitive ELISA Kit, Trial Size
Catalog Number
STA-816-T
Size
32 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$320.00
Product Details

Advanced glycation end products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins. The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent AGE products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage.

The OxiSelect™ N-epsilon-(Carboxymethyl) Lysine Competitive ELISA kit specifically detects CML formation with a high level of sensitivity. This is a Competitive ELISA Kit in which the plate is coated with a CML conjugate.  Standards and unknown samples are added to the plate, followed by incubation with the primary antibody. The CML in the unknown samples and the CML attached to the plate compete for the primary antibody. Higher CML content in unknown samples results in more binding of the antibody to the sample, and thus less antibody binds to the plate. Since the antibody bound to the sample is washed away, higher CML content in samples correlates with a lower signal.

Advanced Glycation End Product Formation Pathways. CML = N-epsilon-(carboxymethyl)lysine. GOLD = glyoxal-derived lysine dimer. CEL = N-epsilon-(1-carboxyethyl)lysine. MOLD = methylglyoxal-derived lysine dimer. DOLD = 3-deoxyglucosone-derived lysine dimer. 3-DG = 3-deoxyglucosone.

Immunoblotting of CML-BSA Control.

Recent Product Citations
  1. Chao, K.C. et al. (2016). Effects of ferric citrate supplementation on advanced glycation end products in a rat model of streptozotocin/nicotinamide-induced diabetes. Mol. Nutr. Food Res. doi:10.1002/mnfr.201600753.
  2. Hansen, L.M. et al. (2016). The receptor for advanced glycation end products impairs collateral formation in both diabetic and non-diabetic mice. Lab. Invest. doi:10.1038/labinvest.2016.113.
  3. Lopez-Moreno, J. et al. (2016). Mediterranean diet supplemented with Coenzyme Q10 modulates the postprandial metabolism of advanced glycation end products in elderly men and women. J. Gerontol. A Biol. Sci. Med. Sci. doi:10.1093/gerona/glw214.
  4. Kuwano, T. et al. (2016). Advanced glycation of high-density lipoprotein and the functionality of aldosterone release in type 2 diabetes. Hyptertension Res. doi:10.1038/hr.2016.141.
  5. Martins, L. S. et al. (2015). Advanced Glycation Endproducts (AGE) evolution after pancreas-kidney transplantation: plasmatic and cutaneous assessments. Oxid Med Cell Longev. 2189582.
  6. Niquet-Léridon, C. et al. (2015). The rehabilitation of raw and brown butters by the measurement of two of the major Maillard products, Nε-Carboxymethyl-Lysine and 5-Hydroxymethylfurfural, with validated chromatographic methods. Food Chemistry.177:361-368.
  7. Huang, T. C. et al. (2014). Increased renal semicarbazide-sensitive amine oxidase activity and methylglyoxal levels in aristolochic acid-induced nephrotoxicity. Life Sci. 114:4-11.
  8. Morgan, P. E. et al. (2014). Perturbation of human coronary artery endothelial cell redox state and NADPH generation by methylglyoxal. PLoS One. 9:e86564.