CML Competitive ELISA

CML Competitive ELISA
  • Detect CML as low as 2.25 ng/mL from a variety of samples
  • CML-modified BSA included as standard
  • Compatible with cell lysates, serum, plasma, purified proteins, and other protein-containing samples


Frequently Asked Questions about this product

General FAQs about Oxidative Stress

Video: Color Development in an ELISA

Email To BuyerPrint this PageCopy Link

Please contact your distributor for pricing.

OxiSelect™ N-epsilon-(Carboxymethyl) Lysine (CML) Competitive ELISA Kit
Catalog Number
96 assays
Manual/Data Sheet Download
SDS Download
OxiSelect™ N-epsilon-(Carboxymethyl) Lysine (CML) Competitive ELISA Kit
Catalog Number
5 x 96 assays
Manual/Data Sheet Download
SDS Download
Product Details

Advanced glycation end products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins. The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent AGE products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage.

The OxiSelect™ N-epsilon-(Carboxymethyl) Lysine Competitive ELISA kit specifically detects CML formation with a high level of sensitivity. This is a Competitive ELISA Kit in which the plate is coated with a CML conjugate.  Standards and unknown samples are added to the plate, followed by incubation with the primary antibody. The CML in the unknown samples and the CML attached to the plate compete for the primary antibody. Higher CML content in unknown samples results in more binding of the antibody to the sample, and thus less antibody binds to the plate. Since the antibody bound to the sample is washed away, higher CML content in samples correlates with a lower signal.

Advanced Glycation End Product Formation Pathways. CML = N-epsilon-(carboxymethyl)lysine. GOLD = glyoxal-derived lysine dimer. CEL = N-epsilon-(1-carboxyethyl)lysine. MOLD = methylglyoxal-derived lysine dimer. DOLD = 3-deoxyglucosone-derived lysine dimer. 3-DG = 3-deoxyglucosone.

Recent Product Citations
  1. Shen, C.Y. et al. (2023). Unveiling the molecular basis of inflamm-aging induced by advanced glycation end products (AGEs)-modified human serum albumin (AGE-HSA) in patients with different immune-mediated diseases. Clin Immunol. 252:109655. doi: 10.1016/j.clim.2023.109655.
  2. Damasiewicz-Bodzek, A. & Nowak, A. (2022). Concentrations of N6-Carboxymethyllysine (CML), N6-Carboxyethyllysine (CEL), and Soluble Receptor for Advanced Glycation End-Products (sRAGE) Are Increased in Psoriatic Patients. Biomolecules. 12(12):1870. doi: 10.3390/biom12121870.
  3. Vaidya, R. et al. (2022). Accumulation of fluorescent advanced glycation end products and carboxymethyl-lysine in human cortical and trabecular bone. Bone Rep. doi: 10.1016/j.bonr.2022.101634.
  4. Imai, Y. et al. (2022). Inhibitory Effects of Parachlorella Beijerinckii Extracts on the Formation of Advanced Glycation End Products and Glycative Stress-Induced Inflammation in an In Vitro Skin Dermis-Like Model. Evid. Based Complementary Altern. Med. doi: 10.1155/2022/8789903.
  5. Yoon, S. et al. (2022). Effect of Cirsium japonicum Flower Extract on Skin Aging Induced by Glycation. Molecules. 27(7):2093. doi: 10.3390/molecules27072093.
  6. Maciejczyk, M. et al. (2022). Oxidation, Glycation, and Carbamylation of Salivary Biomolecules in Healthy Children, Adults, and the Elderly: Can Saliva Be Used in the Assessment of Aging? J Inflamm Res. 15:2051-2073. doi: 10.2147/JIR.S356029.
  7. Jarrete, A.P. et al. (2022). Alterations in pro- and anti-inflammatory mediators are involved in microvascular dysfunction in postmenopausal women with type 2 diabetes mellitus. Braz J Med Biol Res. 55:e11821. doi: 10.1590/1414-431X2021e11821.
  8. Rajamanickam, A. et al. (2021). Diminished circulating levels of angiogenic factors and RAGE ligands in helminth-diabetes comorbidity and reversal following anthelmintic treatment. J Infect Dis. doi: 10.1093/infdis/jiab170.
  9. Pantner, Y. et al. (2021). DJ-1 attenuates the glycation of mitochondrial complex I and complex III in the post-ischemic heart. Sci Rep. 11(1):19408. doi: 10.1038/s41598-021-98722-1.
  10. Shin, S. et al. (2021). Anti-glycation activities of methyl gallate in vitro and in human explants. J Cosmet Dermatol. doi: 10.1111/jocd.14406.
  11. Mahmoud, A.M. & Ali, M.M. (2021) High Glucose and Advanced Glycation End Products Induce CD147-Mediated MMP Activity in Human Adipocytes. Cells. 10(8):2098. doi: 10.3390/cells10082098.
  12. Lazzari, T.K. et al. (2021). Leptin and advanced glycation end products receptor (RAGE) in tuberculosis patients. PLoS One. 16(7):e0254198. doi: 10.1371/journal.pone.0254198.
  13. Ahmad, S. et al. (2021). Gold Nanoparticle-Bioconjugated Aminoguanidine Inhibits Glycation Reaction: An In Vivo Study in a Diabetic Animal Model. Biomed Res Int. doi: 10.1155/2021/5591851.
  14. Chiew, Y. et al. (2021). Tocotrienol-rich vitamin E from palm oil (Tocovid) and its effects in diabetes and diabetic retinopathy: a pilot phase II clinical trial. Asian J. Ophthalmol. 17(4):375-399. doi: 10.35119/asjoo.v17i4.698.
  15. Altomare, A. et al. (2021). In-Depth AGE and ALE Profiling of Human Albumin in Heart Failure: Ex Vivo Studies. Antioxidants (Basel). 10(3):358. doi: 10.3390/antiox10030358.
  16. Li, Y.Y. et al. (2021). Protective effects of dietary carnosine during in-vitro digestion of pork differing in fat content and cooking conditions. J Food Biochem. 45(2):e13624. doi: 10.1111/jfbc.13624.
  17. Chen, Z. et al. (2020). Association of carbamylated high-density lipoprotein with coronary artery disease in type 2 diabetes mellitus: carbamylated high-density lipoprotein of patients promotes monocyte adhesion. J Transl Med. 18(1):460. doi: 10.1186/s12967-020-02623-2.
  18. Merhi, Z. et al. (2020). Perinatal Exposure to High Dietary Advanced Glycation End-Products Affects the Reproductive System in Female Offspring in Mice. Mol Hum Reprod. doi: 10.1093/molehr/gaaa046.
  19. Gutierrez-Mariscal, F.M. et al. (2020). Reduction in Circulating Advanced Glycation End Products by Mediterranean Diet is Associated with Increased Likelihood of type 2 Diabetes Remission in Patients with Coronary Heart Disease: From the Cordioprev Study. Mol Nutr Food Res. doi: 10.1002/mnfr.201901290.
  20. Thornton, K. et al. (2020). Dietary Advanced Glycation End Products (AGEs) could alter ovarian function in mice. Mol Cell Endocrinol. doi: 10.1016/j.mce.2020.110826.
  21. Hernández, C. et al. (2020). The Usefulness of Serum Biomarkers in the Early Stages of Diabetic Retinopathy: Results of the EUROCONDOR Clinical Trial. J Clin Med. 9(4). pii: E1233. doi: 10.3390/jcm9041233.
  22. Velayoudom-Cephise, F.L. et al. (2020). Receptor For Advanced Glycated End Products Modulates Oxidative Stress And Mitochondrial Function In The Soleus Muscle Of High Fat Fed Mice. Appl Physiol Nutr Metab. doi: 10.1139/apnm-2019-0936.
  23. Chen, S.H. et al. (2020). Iron and Advanced Glycation End Products: Emerging Role of Iron in Androgen Deficiency in Obesity. Antioxidants. 9:261. doi: 10.3390/antiox9030261.
  24. Shimizu, Y. et al. (2020). Role of DJ‐1 in Modulating Glycative Stress in Heart Failure. J Am Heart Assoc. 9(4). doi: 10.1161/jaha.119.014691.
  25. de la Cruz-Ares, S. et al. (2020). Endothelial Dysfunction and Advanced Glycation End Products in Patients with Newly Diagnosed Versus Established Diabetes: From the CORDIOPREV Study. Nutrients. 12(1). pii: E238. doi: 10.3390/nu12010238.
  26. Lee, J. et al. (2019). Mitochondrial carnitine palmitoyltransferase 2 is involved in Nε-(carboxymethyl)-lysine-mediated diabetic nephropathy. Pharmacol Res. doi: 10.1016/j.phrs.2019.104600.
  27. Kaburagi, T. et al. (2019). Low-Carbohydrate Diet Inhibits Different Advanced Glycation End Products in Kidney Depending on Lipid Composition but Causes Adverse Morphological Changes in a Non-Obese Model Mice. Nutrients. 11(11). pii: E2801. doi: 10.3390/nu11112801.
  28. Yang, J. et al. (2019). Neutrophil-derived advanced glycation end products-Nε-(carboxymethyl) lysine promotes RIP3-mediated myocardial necroptosis via RAGE and exacerbates myocardial ischemia/reperfusion injury. FASEB J. doi: 10.1096/fj.201900115RR.
  29. Ndidi, U.S. et al. (2019). Effect of N(Epsilon)-(carboxymethyl)lysine on Laboratory Parameters and Its Association with βS Haplotype in Children with Sickle Cell Anemia. Disease Markers. doi: 10.1155/2019/1580485.
  30. Ferron, A.J.T. et al. (2019). Protective Effect of Tomato-Oleoresin Supplementation on Oxidative Injury Recoveries Cardiac Function by Improving β-Adrenergic Response in a Diet-Obesity Induced Model. Antioxidants (Basel). 8(9). pii: E368. doi: 10.3390/antiox8090368.