FAQ: Rapid GST Inclusion Body Solubilization and Renaturation Kit

Q: Will the proteins retrieved with this kit be in their native forms?

A: The proteins retrieved using this kit will be renatured and properly folded, since only correctly folded proteins are able to bind to the glutathione beads.


Q: What is the optimal detergent ratio?

A: Every GST fusion protein will require a different ratio of detergent to properly fold.  We recommend first running a small-scale experiment using each of the detergent ratios listed on Table 1 of the product manual to determine the optimal ratio for your protein.  Once this is determined, the assay can be scaled up.