Q: Does this antibody cross react with EGFP?
A: Our anti-RFP antibody is a monoclonal antibody raised against the N-terminal peptide sequence of DsRed (VNGHEFEIEGEGEGR). Based on the peptide sequence alignment with EGFP, there is some degree of similarity and there may be some cross reactivity when using this antibody in a system where EGFP is overexpressed.
Q: Will this antibody work for immunofluorescence?
A: Yes, we have had customers use this antibody for immunofluorescence using a 1:500 dilution.