Q: Can this kit be used with samples from any species?
A: Yes, our SOD Activity Assay is not species specific and should work with any biological protein sample, because it measures enzymatic activity using a xanthine/xanthine oxidase method and does not depend on protein structure which can vary between species.
Q: Is it possible to test samples that have been stored frozen?
A: Two months at -80ºC generally the longest most antioxidant samples should be stored, but we recommend testing fresh samples wherever possible for the best results.
A: SOD enzymes are classified into three groups: cytosolic Cu/Zn-SOD, mitochondrial Mn-SOD, and extracellular Ec-SOD. Our OxiSelect™ Superoxide Dismutase Activity Assay Kit detects all forms of SOD.
Q: Is it necessary to use inhibitors? If so, which ones should I use?
A: This kit allows for SOD inhibitor screening; however it is not necessary. Based on the literature, the most commonly used SOD inhibitor is Diethyldithiocarbamate.