FAQ: Superoxide Dismutase Activity Assay

Q: Can this kit be used with samples from any species?

A: Yes, our SOD Activity Assay is not species specific and should work with any biological protein sample, because it measures enzymatic activity using a xanthine/xanthine oxidase method and does not depend on protein structure which can vary between species.


Q: Is it possible to test samples that have been stored frozen?

A: Two months at -80ºC generally the longest most antioxidant samples should be stored, but we recommend testing fresh samples wherever possible for the best results.


Q: Does your Superoxide Dismutase Activity Assay detect all forms of superoxide dismutase?

A: SOD enzymes are classified into three groups: cytosolic Cu/Zn-SOD, mitochondrial Mn-SOD, and extracellular Ec-SOD. Our OxiSelect™ Superoxide Dismutase Activity Assay Kit detects all forms of SOD.


Q: Is it necessary to use inhibitors?  If so, which ones should I use?

A: This kit allows for SOD inhibitor screening; however it is not necessary.  Based on the literature, the most commonly used SOD inhibitor is Diethyldithiocarbamate.