Q: Do these cells require culturing on gelatin coated plates?
A: Culturing the SNL cells on gelatin coated plates is optional but it typically is not necessary.
Q: Is it necessary to add LIF to the media?
A: The SNL cell line stably expresses mouse LIF and there is no need to add additional LIF when using these feeder cells for mouse iPS culture.
Q: How many times can these cells be passaged?
A: These cells are not primary cells and can be passaged up to 15 times after thawing without seeing senescence of the cells.
Q: Can I make a stock from these cells?
A: Yes, these cells can be frozen down to keep a stock, similar to any other cell line.