FAQ: SNL Feeder Cells

Q: Do these cells require culturing on gelatin coated plates?

A: Culturing the SNL cells on gelatin coated plates is optional but it typically is not necessary.

 

Q: Is it necessary to add LIF to the media?

A: The SNL cell line stably expresses mouse LIF and there is no need to add additional LIF when using these feeder cells for mouse iPS culture.

 

Q: How many times can these cells be passaged?

A: These cells are not primary cells and can be passaged up to 15 times after thawing without seeing senescence of the cells. 

 

Q: Can I make a stock from these cells?

A: Yes, these cells can be frozen down to keep a stock, similar to any other cell line.