FAQ: Adenovirus Purification

Q: What are the main differences between your two Adenovirus Purification Kits?

A: Although both products use the same principal to purify adenovirus, which is based on virus size and surface charge, there are a couple of differences:

1. #VPK-099 uses a spin column while #VPK-100 uses a syringe filter.

2. #VPK-099 can purify one T75 flask or one 10cm dish per prep, while #VPK-100 can handle up to four flasks or dishes per prep.

 

 

Q: Will these kits work with any strain of adenovirus?

A: Our Adenovirus Purification Kits work based on virus surface charge and virus size and will work with any strain of adenovirus.  Purification of adenoviral lysates results in up to 90% virus recovery while removing cellular debris that can interfere with infection. 

 

 

Q: What is the titer of adenovirus purified with these kits?

A: Our Adenovirus Purification Kits are able to recover at least 90% of adenovirus with a similar purity to CsCl preps.  The titer obtained with these kits is dependent upon the starting titer, so therefore the titer will vary and there is not one expected titer.  We recommend measuring the titer of the crude prep before purification to know exactly the starting titer. 

 

 

Q: Can I store the virus at -80ºC in elution buffer without adding glycerol?

A: It is fine to store the virus at -80ºC in elution buffer without adding glycerol.  Multiple freeze-thaws can kill the virus, so we recommend aliquoting the virus before freezing.

 

FAQs for Specific Adenovirus Purification Kits: