GST Protein Inclusion Body Solubilization

GST Protein Inclusion Body Solubilization
  • Quickly solubilize and renature GST inclusion bodies
  • No lengthy dialysis or dilution steps
  • No pH variation or redox pair involved

 

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Rapid GST Inclusion Body Solubilization and Renaturation Kit
Catalog Number
AKR-110
Size
1 kit
Detection
N/A
Manual/Data Sheet Download
SDS Download
Price
$395.00
Product Details

Recombinant proteins expressed in bacteria often form inclusion bodies, especially when they are expressed at high levels. The Rapid GST Inclusion Body Solubilization and Renaturation Kit is designed to retrieve expressed GST fusion proteins in soluble form after lysis and extraction procedures. Each kit contains sufficient reagents to solubilize and renature up to 5-10 liters of bacterial culture.

GST Inclusion Body Solubilization and Renaturation Flow Chart

Solubilization and Renaturation of GST-RTK fusion protein. GST-RTK expression was induced with 1 mM IPTG at 37ºC for 4 hours. The cell pellet was lysed, and inclusion bodies were solubilized and renatured under different amounts of detergent solubilization solution according to the assay protocol. Lane 1: MW Standard; Lane 2: Whole E. coli lysate; Lanes 3, 7, 11: No detergent; Lanes 4, 8, 12: 32-fold dilution; Lanes 5, 9, 13: 8-fold dilution; Lanes 6, 10, 14: 2-fold dilution.

Recent Product Citations
  1. Gao, Q. et al. (2019). Purification of insoluble GST-fused and GST-cleaved Cav1.2 channel fragment by denaturation and renaturation. Protein Expr Purif. 160:7-10. doi: 10.1016/j.pep.2019.03.013.
  2. Campion, C.G. et al. (2018). COMMD5/HCaRG Hooks Endosomes on Cytoskeleton and Coordinates EGFR Trafficking. Cell Rep. 24(3):670-684.e7. doi: 10.1016/j.celrep.2018.06.056.
  3. Wang, H. et al. (2017). Truncated protein tyrosine phosphatase receptor type O suppresses AKT signaling through IQ motif containing GTPase activating protein 1 and confers sensitivity to bortezomib in multiple myeloma. Oncotarget. 8(69):113858-113873. doi: 10.18632/oncotarget.23017.
  4. Hamel, V. et al. (2017). Identification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis. Curr Biol. 27(16):2486-2498.e6. doi: 10.1016/j.cub.2017.07.011.
  5. Dixon, J. E. et al. (2016). Highly efficient delivery of functional cargoes by the synergistic effect of GAG binding motifs and cell-penetrating peptides. Proc Natl Acad Sci U S A. 113:E291-E299.
  6. Matsuoka, T. et al. (2014). Expression and characterization of honeybee, Apis mellifera, larva chymotrypsin-like protease. Apidologie. doi:10.1007/s13592-014-0313-2.
  7. Keller, D. et al. (2014). Mechanisms of HsSAS-6 assembly promoting centriole formation in human cells. J. Cell Biol. 204:697-712.
  8. Lalani, S. et al. (2013). MCTP2 is a Dosage-Sensitive Gene Required for Cardiac Outflow Tract Development. Hum. Mol. Genet. 10.1093/hmg/ddt283.
  9. Sabbah, M. et al. (2011). CCN5, A Novel Transcriptional Repressor of Transforming Growth Factor-ß Signaling Pathway. Mol. Cell. Biol. 10.1128/MCB.01316-10.