HBsAg ELISA Kit

HBsAg ELISA Kit
  • Measures the Hepatitis B surface antigen (HBsAg) in purified virus or unpurified viral supernatant
  • Detection limit of approximately 1 ng/ml
  • HBsAg standard included

 

Video: Color Development in an ELISA

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QuickTiter™ Hepatitis B Surface Antigen (HBsAg) ELISA Kit
Catalog Number
VPK-5004
Size
96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$875.00
QuickTiter™ Hepatitis B Surface Antigen (HBsAg) ELISA Kit
Catalog Number
VPK-5004-5
Size
5 x 96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$3,795.00
Product Details

The Hepatitis B virus (HBV) causes Hepatitis B, an infection of the liver, and is transmitted through blood or bodily fluids. Markers of HBV infection include the surface antigen (HBsAg), the e antigen (HBeAg), and the core antigen (HBcAg), which are often used for detection.

Our HBsAg ELISA is a sandwich ELISA that is designed for detection and quantitation of Hepatitis B surface antigen in purified virus or unpurified viral supernatant. This assay uses an anti-HBsAg antibody coated plate that binds to any HBsAg present in the sample. A FITC-conjugated anti-HBsAg antibody is then added, which binds to HBsAg sample-antibody complex. This is detected by the addition of an HRP-conjugated anti-FITC antibody.  HBsAg levels in unknown samples are calculated based on the HBsAg standard curve.

Recent Product Citations
  1. Huang, M. et al. (2025). Hepatitis B virus promotes liver cancer by modulating the immune response to environmental carcinogens. Nat Commun. 16(1):5360. doi: 10.1038/s41467-025-60894-z.
  2. Khan, A. et al. (2024). Seroprevalence of Hepatic Viruses and Associated Risk Factors Among Rehabilitated Patients During the Post-Addictive Phase. JHRR. 4(3):1-5. doi: 10.61919/jhrr.v4i3.1370.
  3. Duraisamy, G.S. et al. (2022). Selected ginsenosides interfere efficiently with hepatitis B virus mRNA expression levels and suppress viral surface antigen secretion. Heliyon. 8(9):e10465. doi: 10.1016/j.heliyon.2022.e10465.
  4. Du, N. et al. (2022). CircRNA circBACH1 facilitates hepatitis B virus replication and hepatoma development by regulating the miR-200a-3p/MAP3K2 axis. Histol Histopathol. doi: 10.14670/HH-18-452.
  5. Chauhan, R. et al. (2021). Efficient Inhibition of Hepatitis B Virus Replication and cccDNA Formation by HBV Ribonuclease H Inhibitors During Infection. Antimicrob Agents Chemother. doi: 10.1128/AAC.01460-21.
  6. Domfeh, S.A. et al. (2021). Cryptolepine and Nibima inhibit hepatitis B virus replication. Sci Afr. doi: 10.1016/j.sciaf.2021.e00942.
  7. Le, C. et al. (2021). In Vitro Infection with Hepatitis B Virus Using Differentiated Human Serum Culture of Huh7.5-NTCP Cells without Requiring Dimethyl Sulfoxide. Viruses. 13(1):E97. doi: 10.3390/v13010097.
  8. Wang, W. et al. (2020).  A long-acting 3TC ProTide nanoformulation suppresses HBV replication in humanized mice. Nanomedicine. doi: 10.1016/j.nano.2020.102185.
  9. Zhao, Y. et al. (2019). MiR-3613-3p impairs IFN-induced immune response by targeting CMPK1 in chronic hepatitis B. Infect Genet Evol. 103919. doi: 10.1016/j.meegid.2019.103919.
  10. Hussan, A.A. et al. (2018). Prevelance Of Cholelithiasis In Iraqi Patients with Hepatitis B Infection. Pak. J. Biotechnol. 15(2):391-397.
  11. Kim, S.H. (2017). ELISA for Quantitative Determination of Hepatitis B Virus Surface Antigen. Immune Netw. 17(6):451-459. doi: 10.4110/in.2017.17.6.451.