HCV Core Antigen ELISA

HCV Core Antigen ELISA
  • Measures the core antigen of Hepatitis C virus
  • Sensitive to as low as 1 ng/mL
  • Recombinant HCVcAg standard included


Video: Color Development in an ELISA

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QuickTiter™ HCV Core Antigen ELISA Kit
Catalog Number
96 wells
Manual/Data Sheet Download
SDS Download
Product Details

An anti-HCVcAg monoclonal coating antibody is adsorbed onto a microtiter plate. HCV core antigen present in the sample or standard binds to the antibodies adsorbed on the plate; a FITC-conjugated mouse anti-HCVcAg antibody is added and binds to the antigen captured by the first antibody.

Following incubation and wash steps, a HRP-conjugated mouse anti-FITC antibody is added and binds to the FITC conjugated anti-HCVcAg. Following unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and substrate solution reactive with HRP is added to the wells.

A colored product is formed in proportion to the amount of HCV core antigen present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from recombinant HCV core antigen and sample concentration is then determined.

Recent Product Citations
  1. Rani, S.V. et al. (2023). Detection of Hepatitis C Virus Infection and its Genotypic Characterisation among Hemodialysis Patients by HCV Core Antigen Elisa and RTPCR- A Hospital- Based Prospective Study in a Tertiary Care Hospital in South India. Int J Acad Med Pharm. 5(4): 628-632. doi: 10.47009/jamp.2023.5.4.125.
  2. Kong, L. et al. (2021). The synthetic opioid fentanyl enhances viral replication in vitro. PLoS One. 16(4):e0249581. doi: 10.1371/journal.pone.0249581.
  3. Hammerstad, S.S. et al. (2019). Hepatitis C virus infection of human thyrocytes: metabolic, hormonal, and immunological implications. J Clin Endocrinol Metab. pii: dgz241. doi: 10.1210/clinem/dgz241.
  4. Blackard, J.T. et al. (2019). CCR5 receptor antagonism inhibits hepatitis C virus (HCV) replication in vitro. PLoS One. 14(10):e0224523. doi: 10.1371/journal.pone.0224523.
  5. Plissonnier, M.L. et al. (2019). LARP1 binding to hepatitis C virus particles is correlated with intracellular retention of viral infectivity. Virus Res. doi: 10.1016/j.virusres.2019.197679.
  6. Kumar, S. et al. (2019). MARCH8 Ubiquitinates the Hepatitis C Virus Nonstructural 2 Protein and Mediates Viral Envelopment. Cell Rep. 26(7):1800-1814.e5. doi: 10.1016/j.celrep.2019.01.075.
  7. Hassan, R. et al. (2018). Role of Hepatitis C Virus Core Antigen Assay in Blood Donors Screening at Zagazig University Hospitals. Afro-Egyptian Journal of Infectious and Endemic Diseases. 8(1):48-54. doi: 10.21608/aeji.2018.8735.
  8. Xiao, F. et al. (2018). Interactions between the Hepatitis C Virus Nonstructural 2 Protein and Host Adaptor Proteins 1 and 4 Orchestrate Virus Release. MBio. 9(2). pii: e02233-17. doi: 10.1128/mBio.02233-17.
  9. Blackard, J.T. et al. (2017). A preliminary analysis of hepatitis C virus in pancreatic islet cells. Virol J. 14(1):237. doi: 10.1186/s12985-017-0905-3.
  10. Dvir, R. et al. (2017). Autoimmune hepatitis and occult HCV infection: A prospective single-centre clinical study. Autoimmun Rev. doi: 10.1016/j.autrev.2017.01.015.
  11. Owolabi, O. B. et al. (2015). Hepatitis C virus seroprevalence, antigaenemia and associated risk factors among pregnant women in Nigeria. Ethiop Med J. 53.
  12. Liu, D. et al. (2015). Downregulation of miRNA-30c and miR-203a is associated with Hepatitis C virus core protein-induced epithelial-mesenchymal transition in normal hepatocytes and hepatocellular carcinoma cells. Biochem Biophys Res Commun. doi:10.1016/j.bbrc.2015.07.107.
  13. Jittavisutthikul, S. et al. (2015). Humanized-VHH transbodies that inhibit HCV protease and replication. Viruses. 7:2030-2056.
  14. Amet, T. et al. (2014). BST-2 expression in human hepatocytes is inducible by all three types of interferons and restricts production of hepatitis C virus. Curr Mol Med. 14:349-360.
  15. Kong, L. et al. (2014). HIV infection of hepatocytes results in a modest increase in hepatitis C virus expression in vitro. PLoS One. 9:e83728.
  16. Carpentier, A. et al. (2014). Engrafted human stem cell–derived hepatocytes establish an infectious HCV murine model. J Clin Invest. 124:4953-4964.
  17. Deng, A. et al. (2014). Human immunodeficiency virus type 1 Vpr increases hepatitis C virus RNA replication in cell culture. Virus Res. 184:93-102.