HBV Core Antigen ELISA

  • Measures the core antigen of Hepatitis B virus
  • Sensitive to as low as 1 ng/mL
  • Recombinant HBVcAg standard included

 

Video: Color Development in an ELISA

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QuickTiter™ HBV Core Antigen ELISA Kit
Catalog Number
VPK-150-5
Size
5 x 96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$3,195.00
QuickTiter™ HBV Core Antigen ELISA Kit
Catalog Number
VPK-150
Size
96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$745.00
Product Details

An anti-HBVcAg monoclonal coating antibody is adsorbed onto a microtiter plate. HBV core antigen present in the sample or standard binds to the antibodies adsorbed on the plate; a FITC-conjugated mouse anti-HBVcAg antibody is added and binds to the antigen captured by the first antibody.

Following incubation and wash steps, a HRP-conjugated mouse anti-FITC antibody is added and binds to the FITC conjugated anti-HBVcAg. Following unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and substrate solution reactive with HRP is added to the wells.

A colored product is formed in proportion to the amount of HBV core antigen present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from recombinant HBV core antigen and sample concentration is then determined.