Homocitrulline/Citrulline Assay Kit

  • Suitable for use with plasma, serum, urine, and lysates
  • Detection sensitivity of 37.5 µM homocitrulline or citrulline
  • Homocitrulline and citrulline standards included
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Homocitrulline/Citrulline Assay Kit
Catalog Number
MET-5027
Size
100 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$395.00
Product Details

Homocitrulline is an amino acid that is found in the free form as a metabolite of ornithine. It can also be found on proteins through the process of carbamylation, which involves the reaction of isocyanic acid with lysine residues. High levels of urea in the blood can lead to carbamylation, which has been associated with renal disease.

Citrulline is an amino acid similar in structure to homocitrulline but is one methylene group shorter. Free citrulline is produced from free arginine during nitric oxide production by nitric oxide synthase. It is also synthesized from ornithine and carbamoyl phosphate during the urea cycle. Citrulline can be found in proteins as a result of post translational modifications and it can be formed by the conversion of arginine to citrulline by the enzyme pepdidylarginine deiminase.

Our Homocitrulline/Citrulline Assay Kit detects total homocitrulline/citrulline in plasma, serum, urine, or cell and tissue lysates. Samples are first treated with sodium dodecyl sulfate (SDS) and proteinase K to release free homocitrulline/citrulline residues. Assay reagents are then added, which react with homocitrulline and citrulline to produce a chromophore. The absorbance is read at 540-560nm and the levels of homocitrulline/citrulline are determined based on the provided standard curve.

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