Homocitrulline/Citrulline Assay Kit

Homocitrulline/Citrulline Assay Kit
  • Suitable for use with plasma, serum, urine, and lysates
  • Detection sensitivity of 37.5 µM homocitrulline or citrulline
  • Homocitrulline and citrulline standards included
Email To BuyerPrint this PageCopy Link

Please contact your distributor for pricing.

Homocitrulline/Citrulline Assay Kit
Catalog Number
100 assays
Manual/Data Sheet Download
SDS Download
Product Details

Homocitrulline is an amino acid that is found in the free form as a metabolite of ornithine. It can also be found on proteins through the process of carbamylation, which involves the reaction of isocyanic acid with lysine residues. High levels of urea in the blood can lead to carbamylation, which has been associated with renal disease.

Citrulline is an amino acid similar in structure to homocitrulline but is one methylene group shorter. Free citrulline is produced from free arginine during nitric oxide production by nitric oxide synthase. It is also synthesized from ornithine and carbamoyl phosphate during the urea cycle. Citrulline can be found in proteins as a result of post translational modifications and it can be formed by the conversion of arginine to citrulline by the enzyme pepdidylarginine deiminase.

Our Homocitrulline/Citrulline Assay Kit detects total homocitrulline/citrulline in plasma, serum, urine, or cell and tissue lysates. Samples are first treated with sodium dodecyl sulfate (SDS) and proteinase K to release free homocitrulline/citrulline residues. Assay reagents are then added, which react with homocitrulline and citrulline to produce a chromophore. The absorbance is read at 540-560nm and the levels of homocitrulline/citrulline are determined based on the provided standard curve.

Recent Product Citations
  1. Kumar, V.P. et al. (2023).  PrC-210 Protects against Radiation-Induced Hematopoietic and Intestinal Injury in Mice and Reduces Oxidative Stress. Antioxidants (Basel). 12(7):1417. doi: 10.3390/antiox12071417.
  2. Kiang, J.G. et al. (2022). Female Mice are More Resistant to the Mixed-Field (67% Neutron + 33% Gamma) Radiation-Induced Injury in Bone Marrow and Small Intestine than Male Mice due to Sustained Increases in G-CSF and the Bcl-2/Bax Ratio and Lower miR-34a and MAPK Activation. Radiat Res. doi: 10.1667/RADE-21-00201.1.
  3. Wang, L. et al. (2021). PEG-G-CSF and L-Citrulline Combination Therapy for Mitigating Skin Wound Combined Radiation Injury in a Mouse Model. Radiat Res. doi: 10.1667/RADE-20-00151.1.
  4. Shaughnessy, M.P. et al. (2022). Jejunoileal mucosal growth in mice with a limited microbiome. PLoS One. 17(3):e0266251. doi: 10.1371/journal.pone.0266251.
  5. Lira, A.L.A. et la. (2021). Serum albumin modified by carbamoylation impairs macrophage cholesterol efflux in diabetic kidney disease. J Diabetes Complications. doi: 10.1016/j.jdiacomp.2021.107969.
  6. Ma, Y. et al. (2021). Phage-Display-Derived Peptide Specific to Carbamylated Protein. CS Omega. 6(4):3079-3089. doi: 10.1021/acsomega.0c05481.
  7. Li, X. et al. (2020). IL-18 binding protein (IL-18BP) as a novel radiation countermeasure after radiation exposure in mice. Sci Rep. 10(1):18674. doi: 10.1038/s41598-020-75675-5.
  8. Assefa, A.D. et al. (2020). Fruit Morphology, Citrulline, and Arginine Levels in Diverse Watermelon (Citrullus lanatus) Germplasm Collections. Plants (Basel). 9(9):E1054. doi: 10.3390/plants9091054.
  9. Greig, C.J. et al. (2019). Potentiated serotonin signaling in serotonin re-uptake transporter knockout mice increases enterocyte mass and small intestinal absorptive function. Physiol Rep. 7(21):e14278. doi: 10.14814/phy2.14278.
  10. Park, C.J. et al. (2019). Serum Citrulline Levels Exhibit Circadian Variation and Fluctuations in Relation to Food Intake in Mice. Gastroenterology Res. 12(2):88-92. doi: 10.14740/gr1146.
  11. Sun, J.T. et al. (2016). Cyanate-impaired angiogenesis: association with poor coronary collateral growth in patients with stable angina and chronic total occlusion. J. Am. Heart Assoc. 5:e004700.