Indole is an intermediate in the production of tryptophan in bacteria, fungi, archaea, and some plants. When indole production is required, it can be made from tryptophan breakdown via tryptophanase. In humans, tryptophan catabolism leads to the synthesis of indole and other important bioactive derivatives.
Our Indole Assay Kit is a simple colorimetric assay that measures the total amount of free indole present in urine, fecal, and bacterial samples in a 96-well plate format. Unlike the traditional Kovac's reagent method which is less specific, since it also detects 3-methylindole (skatole), our kit uses a proprietary chemistry to form a colored product from indole, but not from skatole or other derivatives. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, standards and unknown samples.