Leukocyte Endothelium Adhesion Assay

  • Quantify interactions between cells and the endothelium
  • Fully quantitative with no manual cell counting
  • Highly sensitive results on a fluorescence plate reader
Email To BuyerPrint this PageCopy Link
Ordering

Please contact your distributor for pricing.

CytoSelect™ Leukocyte-endothelium Adhesion Assay
Catalog Number
CBA-210
Size
100 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$455.00
Product Details

Leukocyte or tumor cell interactions with vascular endothelium consist of a cascade of processes including the firm attachment of cells to endothelial cell adhesion molecules.

The CytoSelect™ Leukocyte Endothelium Adhesion Assay provides a robust system for the quantitative determination of interactions between leukocytes and the endothelium. Adherent cells can be easily quantified on a fluorescence plate reader.

Assay Principle. Endothelial cells are cultured and form a monolayer, which is treated with desired activator or inhibitor. Meanwhile, leukocytes are labeled with the LeukoTracker™ solution, pelleted, washed and resuspended. Leukocytes are added to the monolayer and incubated. Adherent leukocytes are washed, lysed and quantitated by a fluorometer

Human Monocytic THP-1 Adhesion to HUVEC Monolayer. HUVEC monolayer in a 48-well plate was treated with 1 µM PMA for 12 hours. LeukoTracker™ labeled THP-1 cells were allowed to attach to HUVEC monolayer for 1 hour. Adherent cells were lysed and quantified as described in the assay protocol.

Recent Product Citations
  1. He, M. et al. (2016). miR-483 targeting of CTGF suppresses endothelial-to-mesenchymal transition: therapeutic implications in Kawasaki Disease. Circ. Res. doi:10.1161/CIRCRESAHA.116.310233.
  2. Cao, Q. et al. (2016). Myeloid deletion of α1AMPK exacerbates atherosclerosis in LDL receptor knockout (LDLRKO) mice. Diabetes. 65:1565-1576.
  3. Huang, M. et al. (2015). Niclosamide inhibits the inflammatory and angiogenic activation of human umbilical vein endothelial cells. Inflamm Res. 64:1023-1032.
  4. Shah, D. et al. (2015). C1q deficiency promotes pulmonary vascular inflammation and enhances the susceptibility of the lung endothelium to injury. J Biol Chemdoi:10.1074/jbc.M115.690784.
  5. Campos-Estrada, C. et al. (2015). Simvastatin and benznidazole-mediated prevention of Trypanosoma cruzi-induced endothelial activation: role of 15-epi-lipoxin A4 in the action of simvastatin. 9:e0003770.
  6. Ibrahim, A. S. et al. (2015). A lipidomic screen of hyperglycemia-treated HRECs links 12/15-Lipoxygenase to microvascular dysfunction during diabetic retinopathy via NADPH oxidase. J Lipid Res. 56:599-611.
  7. Cao, Q. et al. (2014). Inhibiting DNA Methylation by 5-Aza-2'-deoxycytidine ameliorates atherosclerosis through suppressing macrophage inflammation.Endocrinology. 155:4925-4938.
  8. Cary, L. H. et al. (2014). Interactions between endothelial cells and T cells modulate responses to mixed neutron/gamma radiation. Radiat Res. 181:592-604.
  9. Burrows, G. G. et al. (2014). Human multipotent adult progenitor cells transcriptionally regulate fucosyltransferase VII. Cytotherapy. 16:566-575.
  10. Kapitsinou, P. P. et al. (2014). Endothelial HIF-2 mediates protection and recovery from ischemic kidney injury. J Clin Invest. 124:2396.
  11. Wu, X. Y. et al. (2014). Regulation of microRNA-155 in endothelial inflammation by targeting nuclear factor (NF)-κB p65.  J Cell Biochem. 115:1928-1936.
  12. Ghoshal, P. et al. (2014). Glycosylation inhibitors efficiently inhibit P-selectin-mediated cell adhesion to endothelial cells. PLoS One. 9:e99363.
  13. Sharma-Walia, N. et al. (2014). The Kaposi's Sarcoma-Associated Herpesvirus (KSHV)-Induced 5-Lipoxygenase-Leukotriene B4 Cascade Plays Key Roles in KSHV Latency, Monocyte Recruitment, and Lipogenesis. J. Virol. 88:2131-2156.
  14. Avan, A. et al. (2013). Norepinephrine Increases NADPH Oxidase-Derived Superoxide in Human Peripheral Blood Mononuclear Cells via α-Adrenergic Receptors. Cancer Res. 73:6745-6756.
  15. Liu,G. et al. (2012). ICAM-1–activated Src and eNOS Signaling Increase Endothelial Cell Surface PECAM-1 Adhesivity and Neutrophil Transmigration. Blood. 120:1942-1952.
  16. Fang, Y. et al. (2012). Site-Specific MicroRNA-92a Regulation of Kruppel-Like Factors 4 and 2 in Atherosusceptible Endothelium. Arterioscler Thromb Vasc Biol.32:979-987.
  17. Curatola, A.M. et al. (2012). Dehydroepiandrosterone (DHEA) Inhibition of Monocyte Binding by Vascular Endothelium is Associated with Sialylation of Neural Cell Adhesion Molecule. Reproductive Sciences 19:86-91.
  18. Wang, Y.L. et al. (2011). Innate Immune Function of the Adherens Junction Protein p120-Catenin in Endothelial Response to Endotoxin. J. Immunol. 186-3180-3187.
  19. Xue, J. et al. (2009). NFkB Regulates Thrombin-Induced ICAM-1 Gene Expression in Cooperation with NFAT by Binding to the Intronic NFkB Site in the ICAM-1 gene. Physiol. Genomics 10.1152/physiolgenomics.00012.2009.