96-Well Leukocyte Endothelium Adhesion Assay

Leukocyte Endothelium Adhesion Assay
  • Quantify interactions between cells and the endothelium
  • Fully quantitative with no manual cell counting
  • Highly sensitive results on a fluorescence plate reader
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CytoSelect™ Leukocyte-endothelium Adhesion Assay
Catalog Number
CBA-210
Size
100 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$475.00
Product Details

Leukocyte or tumor cell interactions with vascular endothelium consist of a cascade of processes including the firm attachment of cells to endothelial cell adhesion molecules.

The CytoSelect™ Leukocyte Endothelium Adhesion Assay provides a robust system for the quantitative determination of interactions between leukocytes and the endothelium. Adherent cells can be easily quantified on a fluorescence plate reader.

Assay Principle. Endothelial cells are cultured and form a monolayer, which is treated with desired activator or inhibitor. Meanwhile, leukocytes are labeled with the LeukoTracker™ solution, pelleted, washed and resuspended. Leukocytes are added to the monolayer and incubated. Adherent leukocytes are washed, lysed and quantitated by a fluorometer

Human Monocytic THP-1 Adhesion to HUVEC Monolayer. HUVEC monolayer in a 48-well plate was treated with 1 µM PMA for 12 hours. LeukoTracker™ labeled THP-1 cells were allowed to attach to HUVEC monolayer for 1 hour. Adherent cells were lysed and quantified as described in the assay protocol.

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