Methylglyoxal (MG) Competitive ELISA

Methylglyoxal (MG) Competitive ELISA
  • Rapid detection and quantitation of MG-H1 (methyl-glyoxal-hydro-imidazolone) protein adducts
  • Provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples


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OxiSelect™ Methylglyoxal (MG) Competitive ELISA Kit
Catalog Number
96 assays
Manual/Data Sheet Download
SDS Download
OxiSelect™ Methylglyoxal (MG) Competitive ELISA Kit
Catalog Number
5 x 96 assays
Manual/Data Sheet Download
SDS Download
Product Details

The OxiSelect™ Methylglyoxal (MG) ELISA Kit is an enzyme immunoassay developed for rapid detection and quantitation of MG-H1 (methyl-glyoxal-hydro-imidazolone) protein adducts.  The quantity of MG adduct in protein samples is determined by comparing its absorbance with that of a known MG-BSA standard curve.  Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples.

Recent Product Citations
  1. Oliveira, A.L. et al. (2021). Metformin abrogates the voiding dysfunction induced by prolonged methylglyoxal intake. Eur J Pharmacol. 910:174502. doi: 10.1016/j.ejphar.2021.174502.
  2. Kim, M. et al. (2021). Ishige okamurae Ameliorates Methylglyoxal-Induced Nephrotoxicity via Reducing Oxidative Stress, RAGE Protein Expression, and Modulating MAPK, Nrf2/ARE Signaling Pathway in Mouse Glomerular Mesangial Cells. Foods. 10(9):2000. doi: 10.3390/foods10092000.
  3. Ragno, V.M. et al. (2021). Morphometric, metabolic, and inflammatory markers across a cohort of client-owned horses and ponies on the insulin dysregulation spectrum. J Equine Vet Sci. doi: 10.1016/j.jevs.2021.103715.
  4. Suh, K.S. et al. (2021). Protective effects of sciadopitysin against methylglyoxal-induced degeneration in neuronal SK-N-MC cells. J Appl Toxicol. doi: 10.1002/jat.4211.
  5. Gutierrez-Mariscal, F.M. et al. (2020). Reduction in Circulating Advanced Glycation End Products by Mediterranean Diet is Associated with Increased Likelihood of type 2 Diabetes Remission in Patients with Coronary Heart Disease: From the Cordioprev Study. Mol Nutr Food Res. doi: 10.1002/mnfr.201901290.
  6. Li, J. et al. (2020). Renal protective effects of empagliflozin via inhibition of EMT and aberrant glycolysis in proximal tubules. JCI Insight. pii: 129034. doi: 10.1172/jci.insight.129034.
  7. Piuri, G. et al. (2020). Methylglyoxal, Glycated Albumin, PAF, and TNF-α: Possible Inflammatory and Metabolic Biomarkers for Management of Gestational Diabetes. Nutrients. 12:479. doi: 10.3390/nu12020479.
  8. Shimizu, Y. et al. (2020). Role of DJ‐1 in Modulating Glycative Stress in Heart Failure. J Am Heart Assoc. 9(4). doi: 10.1161/jaha.119.014691.
  9. de la Cruz-Ares, S. et al. (2020). Endothelial Dysfunction and Advanced Glycation End Products in Patients with Newly Diagnosed Versus Established Diabetes: From the CORDIOPREV Study. Nutrients. 12(1). pii: E238. doi: 10.3390/nu12010238.
  10. Liu, C. et al. (2020). Inhibition of thioredoxin 2 by intracellular methylglyoxal accumulation leads to mitochondrial dysfunction and apoptosis in INS-1 cells. Endocrine. doi: 10.1007/s12020-020-02191-x.
  11. Egawa, T. et al. (2019). The Protective Effect of Brazilian Propolis against Glycation Stress in Mouse Skeletal Muscle. Foods. 8(10). pii: E439. doi: 10.3390/foods8100439.
  12. Do, M.H. et al. (2019). Schizonepeta tenuifolia reduces methylglyoxal-induced cytotoxicity and oxidative stress in mesangial cells. J Funct Foods. doi: 10.1016/j.jff.2019.103531.
  13. Nakamura, T. et al. (2019). Poorly controlled type 2 diabetes with no progression of diabetes-related complications and low levels of advanced glycation end products: A Case report. Medicine (Baltimore). 98(30):e16573. doi: 10.1097/MD.0000000000016573.
  14. Griggs, R.B. et al. (2019). Methylglyoxal and a spinal TRPA1-AC1-Epac cascade facilitate pain in the db/db mouse model of type 2 diabetes. Neurobiol Dis. 127:76-86. doi: 10.1016/j.nbd.2019.02.019.
  15. Shamsaldeen, Y.A. et al. (2019). Dysfunction in nitric oxide synthesis in streptozotocin treated rat aorta and role of methylglyoxal. Eur J Pharmacol. 842:321-328. doi: 10.1016/j.ejphar.2018.10.056.
  16. Simón, L. et al. (2018). Olive oil addition to the high-fat diet reduces methylglyoxal (MG-H1) levels increased in hypercholesterolemic rabbits. Mediterranean Journal of Nutrition and Metabolism. doi: 10.3233/mnm-180229.
  17. Thompson, K. et al. (2018). Advanced glycation end (AGE) product modification of laminin downregulates Kir4.1 in retinal Müller cells. PLoS One. 13(2):e0193280. doi: 10.1371/journal.pone.0193280.
  18. Suh, K.S. et al. (2018). Cytoprotective effects of xanthohumol against methylglyoxal-induced cytotoxicity in MC3T3-E1 osteoblastic cells. J Appl Toxicol. 38:180–192. 
  19. Park, S. et al. (2017). Bariatric Surgery can Reduce Albuminuria in Patients with Severe Obesity and Normal Kidney Function by Reducing Systemic Inflammation. Obes Surg. doi: 10.1007/s11695-017-2940-y.
  20. Suh, K.S. et al. (2017). Magnolol protects pancreatic β-cells against methylglyoxal-induced cellular dysfunction. Chem Biol Interact. 277:101-109. doi: 10.1016/j.cbi.2017.09.014.
  21. Suh, K.S. et al. (2017). Limonene protects osteoblasts against methylglyoxal-derived adduct formation by regulating glyoxalase, oxidative stress, and mitochondrial function. Chem Biol Interact. 278:15-21. doi: 10.1016/j.cbi.2017.10.001.
  22. Suh, K.S. et al. (2017). Deoxyactein protects pancreatic β-cells against methylglyoxal-induced oxidative cell damage by the upregulation of mitochondrial biogenesis. Int. J. Mol. Med. doi:10.3892/ijmm.2017.3018.
  23. Nishimoto S, et al. (2017). Activation of Nrf2 attenuates carbonyl stress induced by methylglyoxal in human neuroblastoma cells: Increase in GSH levels is a critical event for the detoxification mechanism. Biochem Biophys Res Commun. doi: 10.1016/j.bbrc.2
  24. Lopez-Moreno, J. et al. (2016). Mediterranean diet supplemented with Coenzyme Q10 modulates the postprandial metabolism of advanced glycation end products in elderly men and women. J. Gerontol. A Biol. Sci. Med. Sci. doi:10.1093/gerona/glw214.
  25. Ueda, K. et al. (2016). Photodegradation of retinal bisretinoids in mouse models and implications for macular degeneration. Proc Natl Acad Sci U S A.  doi:10.1073/pnas.1524774113.
  26. Morgan, P. E. et al. (2014). Perturbation of human coronary artery endothelial cell redox state and NADPH generation by methylglyoxal. PLoS One. 9:e86564.