Nicotinamide adenine dinucleotide (NAD) consists of two nucleotides, adenine and nicotinamide, connected through their phosphate groups. It is found in all living cells, where its role is to transfer electrons, such as in glycolysis and the citric acid cycle. NAD cycles between an oxidized form (NAD+), which serves as an electron acceptor, and a reduced form (NADH), which donates electrons.
Our NAD+/NADH Assay Kit detects NAD+ and NADH in cell and tissue lysates. Total NAD+/NADH can be detected or samples can be treated with an acid or base treatment to specifically detect NAD+ or NADH. This assay uses an enzymatic cycling reaction that reduces NAD+ to NADH, which then reacts with either a colorimetric or fluorometric probe. NAD+/NADH levels in unknown samples are calculated based on the provided NAD+ standard curve.