Nicotinamide adenine dinucleotide phosphate (NADP) is an enzymatic cofactor involved in metabolic redox and cell signaling reactions. Its main function is electron transfer, where it cycles between the electron donating reduced form (NADPH) and the electron accepting oxidized form (NADP+). The major source of NADPH in animal cells is from the oxidative branch of the pentose phosphate pathway (PPP).
Our NADP+/NADPH Assay Kit detects NADP+ and NADPH in cell and tissue lysates. Total NADP+/NADPH can be detected or samples can be treated with an acid or base treatment to specifically detect NADP+ or NADPH. This assay uses an enzymatic cycling reaction that reduces NADP+ to NADPH, which then reacts with either a colorimetric or fluorometric probe. NADP+/ NADPH levels in unknown samples are calculated based on the provided NADP+ standard curve.