Whether you are investigating reactive species, downstream oxidative damage, or antioxidant capacity, Cell Biolabs delivers the depth, precision and sensitivity required. Our expert assay selection and global distributor network ensure rapid access to assays and ongoing technical support throughout study setup, execution and troubleshooting.
Our oxidative stress assays extend beyond basic research, with relevance for antioxidant research in food, nutraceuticals, cosmetics and beyond. The extensive portfolio is trusted by researchers to deliver consistent, validated performance, supporting sensitive detection and reliable measurement even in complex or archived samples.
Founded by scientists, Cell Biolabs understands the challenges of measuring oxidative stress, including the detection of transient reactive species and its multifactorial complexity. This enables you to confidently address evolving research questions from a single, trusted source.
Understanding oxidative stress: Biology that guides measurement
Oxidative stress occurs when reactive oxygen species (ROS) and reactive nitrogen species (RNS) accumulate beyond the capacity of antioxidants to counteract their effects. This dramatic increase in ROS and RNS drives oxidative modification of cellular biomolecules including lipids, proteins and nucleic acids. Accordingly, oxidative stress is most effectively evaluated using three complementary approaches:
- Directly, through measurement of ROS and RNS.
- Indirectly, by quantifying downstream oxidative damage to biomolecules.
- Functionally, by evaluating antioxidant capacity and resistance to oxidative challenge.
Direct measurement of oxidative stress via ROS and RNS
Measuring ROS and RNS captures the reactive chemical drivers of oxidative stress, revealing those that directly initiate damage to biomolecules, including hydrogen peroxide and hydroxyl radicals, and nitric oxide and peroxynitrite anions, respectively.
OxiSelect™ In Vitro ROS/RNS Assay Kit
Designed for sensitive, direct measurement of total oxidative stress, uniquely capturing and quantifying both ROS and RNS within a single assay. Widely cited in oxidative stress research, it delivers an integrated readout of overall oxidative burden.
- Integrated ROS and RNS measurement in a single assay delivers a system-level view of oxidative burden, reducing reliance on individual markers.
- Detection of multiple ROS and RNS (E.g., hydrogen peroxide, nitric oxide, peroxyl radicals and peroxynitrite anion) in a single assay provides broader insight without the need for multiple assay platforms.
- High analytical sensitivity (10 pM DCF; 40 nM hydrogen peroxide) enables reliable detection of low-level oxidative changes and quantitative comparison across studies.
- Broad sample compatibility (E.g., homogenized tissues, cell culture media, cell lysates, plasma, serum and urine) supporting consistent oxidative stress measurement within and between studies without changing workflows.
Indirect measurement of oxidative stress via protein oxidation and nitration
Oxidative stress induces stable modifications to cellular proteins, with protein carbonylation representing the most prevalent and well-established markers of oxidative protein damage.
OxiSelect™ protein carbonyl ELISA kit
A highly sensitive and reliable tool for detecting protein carbonyls, making it widely trusted in oxidative stress research.
Key features:
- High analytical sensitivity (≥10 µg/mL) enables reliable detection of oxidative protein damage in low-abundance samples supporting studies where sample quantity is limited or dilution cannot be avoided.
- No concentration or precipitation steps minimize sample loss, maximising data yield while reducing workflow variability.
- Broad sample compatibility (E.g., plasma, serum, cell lysates, and purified proteins) supporting consistent oxidative stress measurement within and between studies without changing workflows.
- Standard colorimetric ELISA format simple and cost-effective using common microplate readers with robust, reproducible quantification.
OxiSelect™ ORAC Activity Assay Kit
Widely used in food, nutraceutical, and biological research, offering a rapid functional measure of antioxidant capacity, by quantifying neutralization of peroxyl radicals.
- Rapid results (<2 hours) enable fast experimental turnaround and efficient comparison of antioxidant performance in high-throughput workflows.
- Functional measurement against oxygen radicals enables biologically relevant comparison of antioxidant performance across samples and studies.
- Broad sample compatibility (E.g., plasma, cell fractions, tissue lysates, and solid or aqueous nutritional samples) enables consistent antioxidant evaluation across biological, preclinical, and formulation-based studies using a single assay approach.
- Antioxidant standard ensures reliable normalization and quantitative comparison between experiments and studies.
- Fluorometric detection provides the sensitivity and precision required to reliably compare subtle differences in antioxidant performance.
To explore our full oxidative stress assay portfolio, visit our Oxidative & Cellular Stress product page for additional assays, formats, and product information.