Phagocytosis Assay, Zymosan Substrate

Phagocytosis Assay, Zymosan Substrate
  • Fully quantify phagocytosis with no manual cell counting
  • High-throughput 96-well format
  • Convenient quantitation in a standard microplate reader

NOTE: This assay is suitable only for adherent phagocytes. For suspension cells please use one of our other Phagocytosis Assay Kits with either E. coli or Red Blood Cell substrates.

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CytoSelect™ 96-Well Phagocytosis Assay, Zymosan Substrate
Catalog Number
96 assays
Manual/Data Sheet Download
SDS Download
CytoSelect™ 96-Well Phagocytosis Assay, Zymosan Substrate
Catalog Number
5 x 96 assays
Manual/Data Sheet Download
SDS Download
Product Details

Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". The most common substrates used in phagocytosis assays are erythrocytes (RBCs) and zymosan particles. However, traditional assays require tedious cell counting under a microscope.

Our CytoSelect™ 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

Particle Engulfment with the CytoSelect™ 96-Well Phagocytosis Assay (Zymosan Substrate).

Recent Product Citations
  1. Jang, H. et al. (2022). Specific histamine regulating activity of surface-modified yeast vacuoles by histamine- binding protein and its immune-enhancing effect. Microb Biotechnol. doi: 10.1111/1751-7915.14116.
  2. Narayanaperumal, J. et al. (2022). A randomized double blinded placebo controlled clinical trial for the evaluation of green coffee extract on immune health in healthy adults. J Tradit Complement Med. doi: 10.1016/j.jtcme.2022.01.007.
  3. Naveenchandra. et al. (2021). In-vitro cytotoxicity and phagocytic activity of AQUATURM®-water soluble extract of Curcuma longa, on mouse macrophage RAW 264.7 Cell line. Int J Herb Med. 9(5):38-41.
  4. Kim, S. et al. (2021). Immunostimulatory activity of stem bark of Kalopanax pictus in RAW 264.7 macrophage. J Herb Med. doi: 10.1016/j.hermed.2021.100504.
  5. Park, E. et al. (2021). In Vivo Evaluation of Immune-Enhancing Activity of Red Gamju Fermented by Probiotic Levilactobacillus brevis KU15154 in Mice. Foods. 10(2):253. doi: 10.3390/foods10020253.
  6. Shin, J. et al. (2020). Immunomodulatory Effect of a Salvia plebeia R. Aqueous Extract in Forced Swimming Exercise-induced Mice. Nutrients. 12(8):E2260. doi: 10.3390/nu12082260.
  7. Vay, S.U. et al. (2020). The impact of hyperpolarization-activated cyclic nucleotide-gated (HCN) and voltage-gated potassium KCNQ/Kv7 channels on primary microglia function. J Neuroinflammation. 17(1):100. doi: 10.1186/s12974-020-01779-4.
  8. Zuo, P. et al. (2020). Protease-activated receptor 2 deficiency in hematopoietic lineage protects against myocardial infarction through attenuated inflammatory response and fibrosis. Biochem Biophys Res Commun. doi: 10.1016/j.bbrc.2020.03.077.
  9. Trivedi, M.K. et al. (2020). Solid and liquid state characterization of tetrahydrocurcumin using XRPD, FT-IR, DSC, TGA, LC-MS, GC-MS, NMR and its biological activities. J Pharm Anal. doi: 10.1016/j.jpha.2020.02.005.
  10. Dashdulam, D. et al. (2020). Osteopontin heptamer peptide containing the RGD motif enhances the phagocytic function of microglia. Biochem Biophys Res Commun. pii: S0006-291X(20)30175-3. doi: 10.1016/j.bbrc.2020.01.100.
  11. Rabenstein, M. et al. (2020). Crosstalk between stressed brain cells: direct and indirect effects of ischemia and aglycemia on microglia. J Neuroinflammation. 17(1):33. doi: 10.1186/s12974-020-1697-8.
  12. Abumaree, M.H. et al. (2019). Decidua Basalis Mesenchymal Stem Cells Favor Inflammatory M1 Macrophage Differentiation In Vitro. Cells. 8(2). pii: E173. doi: 10.3390/cells8020173.
  13. Kim, S. et al. (2019). Immune-enhancing screening of fourteen plants on murine macrophage RAW 264.7 cells. Trop J Pharm Res. 18(1): 86. doi: 10.4314/tjpr.v18i1.13.
  14. Al-Kushi, A.G. et al. (2018). Antioxidant effect of royal jelly on immune status of hyperglycemic rats. Phcog Mag. 14:528-33. doi: 10.4103/pm.pm_87_18.
  15. Vay, S.U. et al. (2018). The plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo. J Neuroinflammation. 15(1):226. doi: 10.1186/s12974-018-1261-y.
  16. Trivedi, M.K. et al. (2017). Immunomodulatory potential of nanocurcumin-based formulation. Inflammopharmacol. 25 (6): 609-619.
  17. Sapkota, M. et al. (2016). Malondialdehyde-acetaldehyde-adducted surfactant protein alters macrophage functions through Scavenger Receptor A. Alcoholism Clin. Exp. Res. 40:2563-2572.
  18. Rawat, P. & Spector, S. A. (2016). Development and characterization of a human microglia cell model of HIV-1 infection. J Neurovirol. doi:10.1007/s13365-016-0472-1.
  19. Beringer, P. M. et al. (2015). Rhesus θ-defensin-1 (RTD-1) exhibits in vitro and in vivo activity against cystic fibrosis strains of Pseudomonas aeruginosa. J Antimicrob Chemother. doi: 10.1093/jac/dkv301.
  20. Lee, S. G. et al. (2015). Immunostimulatory polysaccharide isolated from the leaves of Diospyros kaki Thumb modulate macrophage via TLR2Int J Biol Macromol. 79:971-982.
  21. Jung, J. Y. et al. (2015). Lactobacillus sakei K040706 evokes immunostimulatory effects on macrophages through TLR 2-mediated activation. Int Immunopharmacol.  doi:10.1016/j.intimp.2015.05.037.
  22. Zhang, H. et al. (2015). Functional analysis and transcriptomic profiling of iPSC-derived macrophages and their application in modeling mendelian disease. Circ Res.  doi:10.1161/CIRCRESAHA.117.305860.
  23. Fiorcari, S. et al. (2015). Lenalidomide interferes with tumor-promoting properties of nurse-like cells in chronic lymphocytic leukemia. Haematologica. 100:253-262.
  24. Liao, W. T. et al. (2014). Cyclic GMP-dependent protein kinase II is necessary for macrophage M1 polarization and phagocytosis via toll-like receptor 2. J Mol Med (Berl). doi: 10.1007/s00109-014-1236-0.
  25. Kasat, K. et al. (2014). Anti-inflammatory actions of endogenous and exogenous interleukin-10 versus glucocorticoids on macrophage functions of the newly born. J Perinatol. 34:380-385.
  26. Haselow, K. et al. (2013). Bile Acids PKA-Dependently Induce a Switch of the IL-10/IL-12 Ratio and Reduce Proinflammatory Capability of Human Macrophages. J. Leukoc. Biol. 94:1253-1264.
  27. Pierce, L.M. et al. (2012). Effect Of Heavy Metal Tungsten Alloy Particles On Oxidative Product Formation And Phagocytosis By Lung Macrophages. Am. J. Respir. Crit. Care Med. 185:A4666.
  28. Polancec, al.(2012). Azithromycin Drives in Vitro GM-CSF/IL-4-Induced Differentiation of Human Blood Monocytes Toward Dendritic-like Cells with Regulatory Properties. J Leukoc Biol. 91:229-243.