Phagocytosis Assay, Zymosan Substrate

  • Fully quantify phagocytosis with no manual cell counting
  • High-throughput 96-well format
  • Convenient quantitation in a standard microplate reader

NOTE: This assay is suitable only for adherent phagocytes. For suspension cells please use one of our other Phagocytosis Assay Kits with either E. coli or Red Blood Cell substrates.

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CytoSelect™ 96-Well Phagocytosis Assay, Zymosan Substrate
Catalog Number
CBA-224
Size
96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$580.00
CytoSelect™ 96-Well Phagocytosis Assay, Zymosan Substrate
Catalog Number
CBA-224-5
Size
5 x 96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$2,490.00
CytoSelect™ 96-Well Phagocytosis Assay, Zymosan Substrate, Trial Size
Catalog Number
CBA-224-T
Size
20 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$290.00
Product Details

Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". The most common substrates used in phagocytosis assays are erythrocytes (RBCs) and zymosan particles. However, traditional assays require tedious cell counting under a microscope.

Our CytoSelect™ 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

Particle Engulfment with the CytoSelect™ 96-Well Phagocytosis Assay (Zymosan Substrate).

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  5. Jung, J. Y. et al. (2015). Lactobacillus sakei K040706 evokes immunostimulatory effects on macrophages through TLR 2-mediated activation. Int Immunopharmacol.  doi:10.1016/j.intimp.2015.05.037.
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  8. Liao, W. T. et al. (2014). Cyclic GMP-dependent protein kinase II is necessary for macrophage M1 polarization and phagocytosis via toll-like receptor 2. J Mol Med (Berl). doi: 10.1007/s00109-014-1236-0.
  9. Kasat, K. et al. (2014). Anti-inflammatory actions of endogenous and exogenous interleukin-10 versus glucocorticoids on macrophage functions of the newly born. J Perinatol. 34:380-385.
  10. Haselow, K. et al. (2013). Bile Acids PKA-Dependently Induce a Switch of the IL-10/IL-12 Ratio and Reduce Proinflammatory Capability of Human Macrophages. J. Leukoc. Biol. 94:1253-1264.
  11. Pierce, L.M. et al. (2012). Effect Of Heavy Metal Tungsten Alloy Particles On Oxidative Product Formation And Phagocytosis By Lung Macrophages. Am. J. Respir. Crit. Care Med. 185:A4666.
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