D-Amino Acid Assay Kits

D-Amino Acid Assay Kits
  • Measure only free D-amino acids (except D-Glutamate and D-Aspartate); does not detect peptides or proteins
  • Detection sensitivity limit of 1.56 µM
  • Suitable for use with lysates, serum, plasma, urine, and food samples
  • D-Alanine standard included
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D-Amino Acid Assay Kit (Colorimetric)
Catalog Number
MET-5136
Size
100 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$320.00
D-Amino Acid Assay Kit (Fluorometric)
Catalog Number
MET-5137
Size
100 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$320.00
Product Details

Our D-Amino Acid Assay Kits measures free D-Amino Acids (except for D-Glutamate and D-Aspartate) present in foods or biological samples in a 96-well microtiter plate format.   D-amino acids are oxidatively deaminated by D-Amino Acid oxidase into the corresponding α-keto acid, ammonia, and hydrogen peroxide.  The hydrogen peroxide is then detected with a highly specific colorimetric or fluorometric probe.  Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio.  D-amino acid levels in an unknown sample are calculated based on a known D-Alanine standard curve.