CBL-BSA standards or protein samples are added to an Anti-CBL Antibody Coated Plate for 2 hours at 37ºC. The CBL protein adducts present in the sample or standard are probed with an anti-CBL antibody, followed by an HRP conjugated secondary antibody. The protein CBL adduct content in an unknown sample is determined by comparing against a standard curve prepared from CBL-BSA standards.