Protein Carbamylation Sandwich ELISA

Protein Carbamylation Sandwich ELISA
  • Detection sensitivity limit of 1.5 ng/mL of CBL-BSA
  • Rapid detection and quantitation of protein carbamylation
  • Kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples

 

Video: Color Development in an ELISA

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OxiSelect™ Protein Carbamylation Sandwich ELISA Kit
Catalog Number
STA-877
Size
96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$670.00
Product Details

CBL-BSA standards or protein samples are added to an Anti-CBL Antibody Coated Plate for 2 hours at 37ºC.  The CBL protein adducts present in the sample or standard are probed with an anti-CBL antibody, followed by an HRP conjugated secondary antibody.  The protein CBL adduct content in an unknown sample is determined by comparing against a standard curve prepared from CBL-BSA standards.

Recent Product Citations
  1. Brevet, P. et al. (2021). Anti-Carbamylated Fibrinogen Antibodies Might Be Associated With a Specific Rheumatoid Phenotype and Include a Subset Recognizing In Vivo Epitopes of Its γ Chain One of Which Is Not Cross Reactive With Anti-Citrullinated Protein Antibodies. Front Immunol. 12:733511. doi: 10.3389/fimmu.2021.733511.
  2. Lira, A.L.A. et la. (2021). Serum albumin modified by carbamoylation impairs macrophage cholesterol efflux in diabetic kidney disease. J Diabetes Complications. doi: 10.1016/j.jdiacomp.2021.107969.
  3. Santana, M.F.M. et al. (2020). Enrichment of apolipoprotein A-IV and apolipoprotein D in the HDL proteome is associated with HDL functions in diabetic kidney disease without dialysis. Lipids Health Dis. 19(1):205. doi: 10.1186/s12944-020-01381-w.
  4. Bright, R. et al. (2017). The effect of triclosan on posttranslational modification of proteins through citrullination and carbamylation. Clin. Oral Investig. doi: 10.1007/s00784-017-2137-8.
  5. Tajerian, M. et al. (2016). Identification of KRT16 as a target of an autoantibody response in complex regional pain syndrome. Exp, Neurol. 287:14-20.
  6. Sun, J. T. et al. (2016). Increased carbamylation level of HDL in end-stage renal disease: Carbamylated-HDL attenuated endothelial cell function. Am J Physiol Renal Physiol. doi:10.1152/ajprenal.00508.2015.