Protein Carbamylation Sandwich ELISA

CBL-BSA standards or protein samples are added to an Anti-CBL Antibody Coated Plate for 2 hours at 37ºC.  The CBL protein adducts present in the sample or standard are probed with an anti-CBL antibody, followed by an HRP conjugated secondary antibody.  The protein CBL adduct content in an unknown sample is determined by comparing against a standard curve prepared from CBL-BSA standards.

1. Tajerian, M. et al. (2016). Identification of KRT16 as a target of an autoantibody response in complex regional pain syndrome. Exp, Neurol. 287:14-20.
2. Sun, J. T. et al. (2016). Increased carbamylation level of HDL in end-stage renal disease: Carbamylated-HDL attenuated endothelial cell function. Am J Physiol Renal Physiol. doi:10.1152/ajprenal.00508.2015.