Rap1 Activation Assay

Rap Activation Assays
  • Safe non-radioactive assay format
  • Colored agarose beads allow visual check
  • Fast results: 1 hour plus electrophoresis/blotting time
  • Compatible with human, mouse, and rat samples

 

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Rap1 Activation Assay Kit
Catalog Number
STA-406-1
Size
20 assays
Detection
Immunoblot
Manual/Data Sheet Download
SDS Download
Price
$695.00
Product Details

Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1. The precipitated small GTPase is then detected by Western blot using a Rap1-specific antibody included in the kit.

Small GTPase Activation Assay Principle

Recent Product Citations
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  4. Ichikawa H, et al. (2017). Overexpression of exchange protein directly activated by cAMP-1 (EPAC1) attenuates bladder cancer cell migration. Biochem Biophys Res Commun. 495(1):64-70. doi: 10.1016/j.bbrc.2017.10.142.
  5. Kikuchi, R. et al. (2017). Hypercapnia accelerates adipogenesis; a novel role of high CO2 in exacerbating obesity. Am. J. Respir. Cell Mol. Biol. doi:10.1065/rcmb-2016-0278OC.
  6. Czikora, A. et al. (2016). Structural basis for the failure of the C1 domain of Ras guanine nucleotide releasing protein 2 (RasGRP2) to bind phorbol ester with high affinity. J Biol Chem. 291:11133-11147.
  7. Ishiguro, K. et al. (2014). Suppressive action of acetate on interleukin-8 production via tubulin-α acetylation.  Immunol Cell Biol. 92:624-630.