S-Adenosylhomocysteine (SAH) ELISA Kit

S-Adenosylhomocysteine (SAH) ELISA Kit
  • Detection sensitivity limit of 0.2 µM SAH
  • Suitable for use with plasma, serum, lysates, and other biological fluid samples
  • SAH standard included


Video: Color Development in an ELISA

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S-Adenosylhomocysteine (SAH) ELISA Kit
Catalog Number
96 assays
Manual/Data Sheet Download
SDS Download
Product Details

S-adenosylhomocysteine (SAH) is generated through the donation of a methyl group from S-adenosylmethionine (SAM) to various biomolecules. SAH is then hydrolyzed to homocysteine and adenosine, which is converted to methionine, and ultimately back to SAM. SAH can act as a methylation inhibitor, therefore the SAM/SAH ratio, or methylation potential, is important for maintaining proper methylation in the cell.

Our S-adenosylhomocysteine (SAH) ELISA is designed for detection and quantitation of SAH in plasma, serum, lysates, or biological fluid samples. This assay is a competitive ELISA where samples and an anti-SAH antibody are added to a plate coated with a SAH conjugate. The sample and conjugate compete for antibody binding, which generates a reverse curve.  Samples with high SAH levels will bind the majority of the antibody, which gets washed away and results in a low OD, while samples with low SAH levels will leave more antibody available to bind to the conjugate, producing a high signal.