S-Adenosylmethionine (SAM) and S-Adenosylhomocysteine (SAH) ELISA Combo Kit

S-Adenosylmethionine (SAM) and S-Adenosylhomocysteine (SAH) ELISA Combo Kit
  • Detection sensitivity limit of 7ng/mL SAH-BSA and 185ng/ml SAM-BSA
  • Suitable for use with plasma, serum, lysates, and other biological fluid samples
  • SAH-BSA and SAM-BSA standards included

 

Video: Color Development in an ELISA

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S-Adenosylmethionine (SAM) and S-Adenosylhomocysteine (SAH) ELISA Combo Kit
Catalog Number
STA-671-C
Size
96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$745.00
Product Details

S-adenosylmethionine (SAM) is a substrate for methyl transfers to various biomolecules that yields S-adenosylhomocysteine (SAH). SAH is then hydrolyzed to homocysteine and adenosine, which is converted to methionine, and ultimately back to SAM. SAH can act as a methylation inhibitor, therefore the SAM/SAH ratio, or methylation potential, is important for maintaining proper methylation in the cell.

Our S-Adenosylmethionine (SAM) and  S-Adenosylhomocysteine (SAH)  ELISA Combo Kit is designed for detection and quantitation of both SAM and SAH in plasma, serum, lysates, or biological fluid samples. This assay is a competitive ELISA where samples and an anti-SAM or anti-SAH antibody are added to a plate coated with a SAM or SAH conjugate. The sample and conjugate compete for antibody binding, which generates a reverse curve.  Samples with high SAM and SAH levels will bind the majority of the antibody, which gets washed away and results in a low OD, while samples with low SAM and SAH levels will leave more antibody available to bind to the conjugate, producing a high signal.

Recent Product Citations
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  3. Montenegro, M.F. et al. (2020). PRMT1-dependent methylation of BRCA1 contributes to the epigenetic defense of breast cancer cells against ionizing radiation. Sci Rep. 10(1):13275. doi: 10.1038/s41598-020-70289-3.
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  5. Kang, Y. et al. (2019). Unraveling the role of quorum sensing-dependent metabolic homeostasis of the activated methyl cycle in a cooperative population of Burkholderia glumae. Sci Rep. 9(1):11038. doi: 10.1038/s41598-019-47460-6.
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