S-Adenosylmethionine (SAM) ELISA Kit

S-Adenosylmethionine (SAM) ELISA Kit
  • Detection sensitivity limit of 0.625 nM SAM
  • Suitable for use with plasma, serum, lysates, and other biological fluid samples
  • SAM standard included


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S-Adenosylmethionine (SAM) ELISA Kit
Catalog Number
96 assays
Manual/Data Sheet Download
SDS Download
Product Details

S-adenosylmethionine (SAM) is a substrate for methyl transfers to various biomolecules that yields S-adenosylhomocysteine (SAH). SAH is then hydrolyzed to homocysteine and adenosine, which is converted to methionine, and ultimately back to SAM. SAH can act as a methylation inhibitor, therefore the SAM/SAH ratio, or methylation potential, is important for maintaining proper methylation in the cell.

Our S-adenosylmethionine (SAM) ELISA is designed for detection and quantitation of SAM in plasma, serum, lysates, or biological fluid samples. This assay is a competitive ELISA where samples and an anti-SAM antibody are added to a plate coated with a SAM conjugate. The sample and conjugate compete for antibody binding, which generates a reverse curve.  Samples with high SAM levels will bind the majority of the antibody, which gets washed away and results in a low OD, while samples with low SAM levels will leave more antibody available to bind to the conjugate, producing a high signal.

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