SNL 76/7 Passage-Independent Feeder Cells

SNL 76/7 Feeder Cells are clonally derived from a mouse fibrobast STO cell line which has been transformed with neomycin resistance and murine LIF genes. This immortalized cell line has been shown to be useful as a feeder cell for ES cell growth as well as for culture of human and mouse iPS cells.

1. Shiba, Y. et al. (2016). Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts. Nature 10.1038/nature19815.
2. Mitzelfelt, K. A. et al. (2016). Human 343delT HSPB5 chaperone associated with early-onset skeletal myopathy causes defects in protein solubility. J Biol Chem. doi:10.1074/jbc.M116.730481.
3. Saito, H. et al. (2016). Adoptive transfer of CD8+ T cells generated from induced pluripotent stem cells triggers regressions of large tumors along with immunological memory. Cancer Res. doi:10.1158/0008-5472.CAN-15-1742.
4. Wu, D. T. & Roth, M. J. (2014). MLV based viral-like-particles for delivery of toxic proteins and nuclear transcription factors. Biomaterials. 35:8416-8426.
5. Takenaka-Ninagawa, N. et al. (2014). Generation of rat-induced pluripotent stem cells from a new model of metabolic syndrome. PLoS One.  9:e104462.
6. Piovan, C. et al. (2014). Generation of mouse lines conditionally over-expressing microRNA using the Rosa26-Lox-Stop-Lox system. Methods Mol Biol. 1194:203-224.
7. Kuroda, T. et al. (2014). In vitro detection of residual undifferentiated cells in retinal pigment epithelial cells derived from human induced pluripotent stem cells. Methods Mol Biol. 1210:183-192.
8. Graversen, V. K. & Chavala, S. H. (2014). Induced pluripotent stem cells: generation, characterization, and differentiation-methods and protocols.Methods Mol Biol.10.1007/7651_2014_148
9. Ni, A. et al. (2014). Sphere formation permits Oct4 reprogramming of ciliary body epithelial cells into induced pluripotent stem cells. Stem Cells Dev. 23:3065-3071.
10. Yi, L. et al. (2012). Multiple Roles of p53-Related Pathways in Somatic Cell Reprogramming and Stem Cell Differentiation. Cancer Res. 72: 5635-5645.